Norian L A, Rosenbusch R F
Department of Microbiology, Immunology and Preventive Medicine, Iowa State University, Ames 50011.
Comp Immunol Microbiol Infect Dis. 1993 Apr;16(2):113-22. doi: 10.1016/0147-9571(93)90003-n.
The effect of conjunctival Mycoplasma bovoculi infection on local and systemic natural killer activity was studied in cattle. A standard in vitro assay against the mouse tumor cell line, YAC-1, was used to measure increases in effector cell activity. Local responses in the eye were investigated by examination of stained exfoliative conjunctival cells and tissue biopsies. An increase in systemic cytolytic activity was seen in infected calves, and maximal values were observed on the second day post infection (DPI). An elevation in the number of large granular lymphocytes was noted in the conjunctival samples, and maximal numbers were again observed at 2 days post infection. It is proposed that natural killer cell activity may play a role in the initial host responses of cattle to M. bovoculi infection.
在牛身上研究了结膜牛支原体感染对局部和全身自然杀伤活性的影响。采用针对小鼠肿瘤细胞系YAC-1的标准体外试验来测量效应细胞活性的增加。通过检查染色的脱落结膜细胞和组织活检来研究眼部的局部反应。在感染的犊牛中观察到全身细胞溶解活性增加，在感染后第二天（DPI）观察到最大值。在结膜样本中注意到大颗粒淋巴细胞数量增加，在感染后2天再次观察到最大值。有人提出，自然杀伤细胞活性可能在牛对牛支原体感染的初始宿主反应中起作用。
