The Guangxi Key Laboratory of Theory & Technology for Environmental Pollution Control, College of Environmental Science & Engineering, Guilin University of Technology, Guilin 541004, PR China; Shandong Provincial Key Laboratory of Water and Soil Conservation and Environmental Protection, School of Resource and Environmental Sciences, Linyi University, Shandong Province, Shuangling Road, Linyi 276005, PR China.
The Guangxi Key Laboratory of Theory & Technology for Environmental Pollution Control, College of Environmental Science & Engineering, Guilin University of Technology, Guilin 541004, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Sep 5;297:122702. doi: 10.1016/j.saa.2023.122702. Epub 2023 Apr 4.
Recently, growing concern has been paid to the toxicity of additives in food. The present study investigated the interaction of two commonly used food colorants, quinoline yellow (QY) and sunset yellow (SY), with catalase and trypsin under physiological conditions by fluorescence, isothermal titration calorimetry (ITC), ultraviolet-vis absorption, synchronous fluorescence techniques as well as molecular docking. Based on the fluorescence spectra and ITC data, both QY and SY could significantly quench the intrinsic fluorescence of catalase or trypsin spontaneously to form a moderate complex driven by different forces. Additionally, the thermodynamics results demonstrated QY bind more tightly to both catalase and trypsin than SY, suggesting QY poses more of a threat to two enzymes than SY. Furthermore, the binding of two colorants could not only lead to the conformational and microenvironmental alterations of both catalase and trypsin, but also inhibit the activity of two enzymes. This study provides an important reference for understanding the biological transportation of synthetic food colorants in vivo, and enhancing their risk assessment on food safety.
最近，人们越来越关注食品添加剂的毒性。本研究通过荧光、等温热力学滴定（ITC）、紫外-可见吸收和同步荧光技术以及分子对接，在生理条件下研究了两种常用食品着色剂喹啉黄（QY）和日落黄（SY）与过氧化氢酶和胰蛋白酶的相互作用。基于荧光光谱和 ITC 数据，QY 和 SY 均可显著猝灭过氧化氢酶或胰蛋白酶的固有荧光，自发形成由不同力驱动的中等复合物。此外，热力学结果表明，QY 与两种酶的结合比 SY 更紧密，这表明 QY 对两种酶的威胁比 SY 更大。此外，两种着色剂的结合不仅会导致过氧化氢酶和胰蛋白酶的构象和微观环境发生变化，还会抑制两种酶的活性。本研究为理解体内合成食用色素的生物运输提供了重要参考，并增强了对食品安全的风险评估。
