Faculty of Chemistry, Sensor and Biosensor Research Center (SBRC) & Nanoscience and Nanotechnology Research Center (NNRC), Razi University, P.O. Box 67149, Kermanshah, Islamic Republic of Iran.
Mol Biol Rep. 2012 Dec;39(12):10045-51. doi: 10.1007/s11033-012-1873-8. Epub 2012 Jul 7.
The use of food dyes is at least controversial due to their essential role. Synthetic color food additives occupy an important place in the food industry. Moreover many of them have been related to health problems mainly in children that are considered the most vulnerable group. The purpose of this work is to present spectrophotometric methods to analyze the interaction of native calf thymus DNA (CT-DNA) with sunset yellow (SY) at physiological pH. Considerable hyperchromism and no red shift with an intrinsic binding constant of 7 × 10(4 )M(-1) were observed in UV absorption band of SY. Binding constants of DNA with complex were calculated at different temperatures. Slow increase in specific viscosity of DNA, induced circular dichroism spectral changes, and no significant changes in the fluorescence of neutral red-DNA solutions in the presence of SY suggest that this molecule interacts with CT-DNA via groove binding mode. Furthermore, the enthalpy and entropy of the reaction between SY and CT-DNA showed that the reaction is exothermic and enthalpy favored (∆H = -58.19 kJ mol(-1); ΔS = -274.36 kJ mol(-1) ) which are other evidences to indicate that van der Waals interactions and hydrogen bonding are the main running forces in the binding of the mentioned molecule and mode of interaction with DNA.
由于其重要作用，食品染料的使用至少存在争议。合成色素食品添加剂在食品工业中占有重要地位。此外，许多食品添加剂已被证明与健康问题有关，尤其是儿童，他们被认为是最脆弱的群体。本工作旨在介绍分光光度法来分析日落黄（SY）与天然小牛胸腺 DNA（CT-DNA）在生理 pH 值下的相互作用。在 SY 的紫外吸收带中观察到相当大的增色效应和没有红移，且固有结合常数为 7×10(4 )M(-1)。在不同温度下计算了 DNA 与配合物的结合常数。DNA 的比粘度缓慢增加，诱导圆二色性光谱变化，以及中性红-DNA 溶液在 SY 存在下的荧光没有显著变化，表明该分子通过沟槽结合模式与 CT-DNA 相互作用。此外，SY 与 CT-DNA 之间反应的焓变和熵变表明该反应是放热的，焓变有利（∆H = -58.19 kJ mol(-1)；ΔS = -274.36 kJ mol(-1)），这是其他表明范德华相互作用和氢键是该分子与 DNA 结合的主要驱动力和相互作用模式的证据。
