Department of Genetic Engineering, College of Engineering & Technology (CET), SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.
Computational Biology Laboratory, Department of Genetic Engineering, College of Engineering & Technology (CET), SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.
Biodegradation. 2023 Aug;34(4):383-403. doi: 10.1007/s10532-023-10028-3. Epub 2023 Apr 14.
Plastic accumulation has become a serious environmental threat. Mitigation of plastic is important to save the ecosystem of our planet. With current research being focused on microbial degradation of plastics, microbes with the potential to degrade polyethylene were isolated in this study. In vitro studies were performed to define the correlation between the degrading capability of the isolates and laccase, a common oxidase enzyme. Instrumental analyses were used to evaluate morphological and chemical modifications in polyethylene, which demonstrated a steady onset of the degradation process in case of both isolates, Pseudomonas aeruginosa O1-P and Bacillus cereus O2-B. To understand the efficiency of laccase in degrading other common polymers, in silico approach was employed, for which 3D structures of laccase in both the isolates were constructed via homology modeling and molecular docking was performed, revealing that the enzyme laccase can be exploited to degrade a wide range of polymers.
塑料积累已成为严重的环境威胁。减轻塑料污染对于拯救我们星球的生态系统至关重要。鉴于目前的研究重点是微生物对塑料的降解，本研究中分离出了具有降解聚乙烯潜力的微生物。进行了体外研究以确定分离物与漆酶之间的降解能力的相关性，漆酶是一种常见的氧化酶。仪器分析用于评估聚乙烯的形态和化学修饰，这表明在两种分离物，铜绿假单胞菌 O1-P 和蜡状芽孢杆菌 O2-B 的情况下，降解过程都稳定开始。为了了解漆酶在降解其他常见聚合物方面的效率，采用了计算方法，通过同源建模构建了两种分离物中漆酶的 3D 结构，并进行了分子对接，结果表明，漆酶可以用于降解广泛的聚合物。
