旋毛虫排泄分泌抗原对小鼠变应性哮喘的干预作用。

Intervention effects of Trichinella spiralis excretory-secretory antigens on allergic asthma in mice.

机构信息

Heilongjiang Provincial Key Laboratory of Zoonosis, College of Veterinary Medicine, Northeast Agricultural University, 600 Changjiang Street, Harbin 150030, China.

出版信息

Int Immunopharmacol. 2023 Jun;119:110101. doi: 10.1016/j.intimp.2023.110101. Epub 2023 Apr 12.

DOI:10.1016/j.intimp.2023.110101

PMID:37058749

Abstract

Allergic asthma is a chronic, heterogeneous and inflammatory respiratory disease, and there are few medicines at present. An increasing number of studies indicate that Trichinella spiralis (T. spiralis) and its excretory-secretory (ES) antigens are inflammatory modulator. Therefore, this study focused on the effects of T. spiralis ES antigens on allergic asthma. Asthma model was established by sensitizing mice with ovalbumin antigen (OVA) and aluminum hydroxide (Al[OH]), the asthmatic mice were interfered using T. spiralis 43 kDa protein (Ts43), T. spiralis 49 kDa protein (Ts49), and T. spiralis 53 kDa protein (Ts53), the important components of ES antigens, to establish ES antigens intervention models. Then, asthma symptom changes, weight changes, and lung inflammation of mice were evaluated. The results showed that ES antigens could relieve symptoms, weight loss, and lung inflammation caused by asthma in the mice, and the effect of combined intervention of Ts43, Ts49, and Ts53 was better. Finally, the effects of ES antigens on type 1 helper T (Th1) and type 2 helper T (Th2) immune responses, and the differentiation direction of T lymphocytes in mice were discussed by detecting Th1 and Th2 cell-related factors and the ratio of CD/CD T cells. The results suggested that the ratio of CD/CD T cells decreased and the ratio of Th1/Th2 cells increased. In conclusion, this study indicated that T. spiralis ES antigens could mitigate allergic asthma in the mice by changing the differentiation direction of CD and CD T cells and regulating the imbalance of Th1/Th2 cells ratio.

摘要

变应性哮喘是一种慢性、异质性和炎症性呼吸系统疾病，目前药物治疗选择有限。越来越多的研究表明，旋毛虫（Trichinella spiralis）及其排泄分泌（ES）抗原是炎症调节剂。因此，本研究聚焦于旋毛虫 ES 抗原对变应性哮喘的影响。通过用卵清蛋白抗原（OVA）和氢氧化铝（Al[OH]）致敏小鼠建立哮喘模型，用旋毛虫 43 kDa 蛋白（Ts43）、旋毛虫 49 kDa 蛋白（Ts49）和旋毛虫 53 kDa 蛋白（Ts53）干预哮喘小鼠，这三种蛋白是 ES 抗原的重要成分，建立 ES 抗原干预模型。然后，评估哮喘模型小鼠的哮喘症状变化、体重变化和肺部炎症情况。结果表明，ES 抗原可以缓解哮喘引起的症状、体重减轻和肺部炎症，Ts43、Ts49 和 Ts53 的联合干预效果更好。最后，通过检测 Th1 和 Th2 细胞相关因子以及 CD/CD T 细胞的比值，探讨 ES 抗原对 1 型辅助 T（Th1）和 2 型辅助 T（Th2）免疫应答以及小鼠 T 淋巴细胞分化方向的影响。结果表明，CD/CD T 细胞比值下降，Th1/Th2 细胞比值升高。综上所述，本研究表明，旋毛虫 ES 抗原通过改变 CD 和 CD T 细胞的分化方向以及调节 Th1/Th2 细胞比值失衡，可减轻哮喘小鼠的过敏性哮喘。