• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

删除第一个糖基化位点可促进拉萨病毒糖蛋白介导的膜融合。

Deletion of the first glycosylation site promotes Lassa virus glycoprotein-mediated membrane fusion.

机构信息

State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430207, China; University of the Chinese Academy of Sciences, Beijing, 100049, China.

State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430207, China.

出版信息

Virol Sin. 2023 Jun;38(3):380-386. doi: 10.1016/j.virs.2023.04.003. Epub 2023 Apr 12.

DOI:10.1016/j.virs.2023.04.003
PMID:37059226
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10311258/
Abstract

The Lassa virus (LASV) is endemic in West Africa and causes severe hemorrhagic Lassa fever in humans. The glycoprotein complex (GPC) of LASV is highly glycosylation-modified, with 11 ​N-glycosylation sites. All 11 N-linked glycan chains play critical roles in GPC cleavage, folding, receptor binding, membrane fusion, and immune evasion. In this study, we focused on the first glycosylation site because its deletion mutant (N79Q) results in an unexpected enhanced membrane fusion, whereas it exerts little effect on GPC expression, cleavage, and receptor binding. Meanwhile, the pseudotype virus bearing GPC was more sensitive to the neutralizing antibody 37.7H and was attenuated in virulence. Exploring the biological functions of the key glycosylation site on LASV GPC will help elucidate the mechanism of LASV infection and provide strategies for the development of attenuated vaccines against LASV infection.

摘要

拉沙病毒（LASV）在西非流行，可导致人类发生严重的出血性拉沙热。LASV 的糖蛋白复合物（GPC）高度糖基化修饰，具有 11 个 N-糖基化位点。所有 11 个 N 连接聚糖链在 GPC 裂解、折叠、受体结合、膜融合和免疫逃逸中都发挥着关键作用。在这项研究中，我们专注于第一个糖基化位点，因为其缺失突变体（N79Q）会导致意想不到的增强的膜融合，而对 GPC 的表达、裂解和受体结合几乎没有影响。同时，携带 GPC 的假型病毒对中和抗体 37.7H 更加敏感，且毒力减弱。探索 LASV GPC 上关键糖基化位点的生物学功能将有助于阐明 LASV 感染的机制，并为开发针对 LASV 感染的减毒疫苗提供策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d332/10311258/0580fdbff203/figs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d332/10311258/81e6000d3114/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d332/10311258/dc43c98c348d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d332/10311258/2e39f3f1fc60/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d332/10311258/970b3633f033/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d332/10311258/a2d73c1fc35f/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d332/10311258/c8257625ef39/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d332/10311258/0580fdbff203/figs1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d332/10311258/81e6000d3114/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d332/10311258/dc43c98c348d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d332/10311258/2e39f3f1fc60/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d332/10311258/970b3633f033/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d332/10311258/a2d73c1fc35f/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d332/10311258/c8257625ef39/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d332/10311258/0580fdbff203/figs1.jpg

相似文献

1
Deletion of the first glycosylation site promotes Lassa virus glycoprotein-mediated membrane fusion.删除第一个糖基化位点可促进拉萨病毒糖蛋白介导的膜融合。
Virol Sin. 2023 Jun;38(3):380-386. doi: 10.1016/j.virs.2023.04.003. Epub 2023 Apr 12.
2
A Lassa Fever Live-Attenuated Vaccine Based on Codon Deoptimization of the Viral Glycoprotein Gene.基于病毒糖蛋白基因密码子去优化的拉沙热减毒活疫苗。
mBio. 2020 Feb 25;11(1):e00039-20. doi: 10.1128/mBio.00039-20.
3
Effects of N-Linked Glycan on Lassa Virus Envelope Glycoprotein Cleavage, Infectivity, and Immune Response.N-连接聚糖对拉沙病毒包膜糖蛋白切割、感染性和免疫应答的影响。
Virol Sin. 2021 Aug;36(4):774-783. doi: 10.1007/s12250-021-00358-y. Epub 2021 Mar 10.
4
Delineating the mechanism of anti-Lassa virus GPC-A neutralizing antibodies.阐明抗拉沙病毒 GPC-A 中和抗体的作用机制。
Cell Rep. 2022 May 24;39(8):110841. doi: 10.1016/j.celrep.2022.110841.
5
Comprehensive Interactome Analysis Reveals that STT3B Is Required for N-Glycosylation of Lassa Virus Glycoprotein.全面互作组分析揭示 STT3B 是拉沙病毒糖蛋白 N-糖基化所必需的。
J Virol. 2019 Nov 13;93(23). doi: 10.1128/JVI.01443-19. Print 2019 Dec 1.
6
Lassa virus-like particles displaying all major immunological determinants as a vaccine candidate for Lassa hemorrhagic fever.展示所有主要免疫决定簇的拉沙病毒样颗粒，可用作拉沙热出血热的候选疫苗。
Virol J. 2010 Oct 20;7:279. doi: 10.1186/1743-422X-7-279.
7
Lassa virus glycoprotein complex review: insights into its unique fusion machinery.拉沙病毒糖蛋白复合物综述：深入了解其独特的融合机制。
Biosci Rep. 2022 Feb 25;42(2). doi: 10.1042/BSR20211930.
8
Neutralizing Antibodies against Lassa Virus Lineage I.抗拉萨病毒 I 系的中和抗体。
mBio. 2022 Aug 30;13(4):e0127822. doi: 10.1128/mbio.01278-22. Epub 2022 Jun 22.
9
Structural conservation of Lassa virus glycoproteins and recognition by neutralizing antibodies.拉沙病毒糖蛋白的结构保守性及其被中和抗体识别。
Cell Rep. 2023 May 30;42(5):112524. doi: 10.1016/j.celrep.2023.112524. Epub 2023 May 18.
10
Alphavirus vector-based replicon particles expressing multivalent cross-protective Lassa virus glycoproteins.基于阿尔巴病毒载体的复制子颗粒表达具有多价交叉保护作用的拉沙病毒糖蛋白。
Vaccine. 2018 Jan 29;36(5):683-690. doi: 10.1016/j.vaccine.2017.12.046. Epub 2017 Dec 26.

引用本文的文献

1
Identification of residues in Lassa virus glycoprotein 1 involved in receptor switch.鉴定拉沙病毒糖蛋白 1 中涉及受体转换的残基。
Virol Sin. 2024 Aug;39(4):600-608. doi: 10.1016/j.virs.2024.06.001. Epub 2024 Jun 6.

本文引用的文献

1
Screening and Identification of Lassa Virus Entry Inhibitors from a Fragment-Based Drug Discovery Library.基于片段的药物发现文库筛选和鉴定拉沙病毒进入抑制剂。
Viruses. 2022 Nov 27;14(12):2649. doi: 10.3390/v14122649.
2
Delineating the mechanism of anti-Lassa virus GPC-A neutralizing antibodies.阐明抗拉沙病毒 GPC-A 中和抗体的作用机制。
Cell Rep. 2022 May 24;39(8):110841. doi: 10.1016/j.celrep.2022.110841.
3
Structure and receptor recognition by the Lassa virus spike complex.拉沙病毒刺突复合体的结构与受体识别
Nature. 2022 Mar;603(7899):174-179. doi: 10.1038/s41586-022-04429-2. Epub 2022 Feb 16.
4
2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales.2021 年门 Negarnaviricota（Riboviria：Orthornavirae）的分类学更新，包括大目 Bunyavirales 和 Mononegavirales。
Arch Virol. 2021 Dec;166(12):3513-3566. doi: 10.1007/s00705-021-05143-6.
5
Special Issue "Arenaviruses 2020".专刊：2020 年沙粒病毒
Viruses. 2021 Apr 18;13(4):703. doi: 10.3390/v13040703.
6
Effects of N-Linked Glycan on Lassa Virus Envelope Glycoprotein Cleavage, Infectivity, and Immune Response.N-连接聚糖对拉沙病毒包膜糖蛋白切割、感染性和免疫应答的影响。
Virol Sin. 2021 Aug;36(4):774-783. doi: 10.1007/s12250-021-00358-y. Epub 2021 Mar 10.
7
Glycoprotein N-linked glycans play a critical role in arenavirus pathogenicity.糖蛋白 N-连接聚糖在沙粒病毒致病性中起着关键作用。
PLoS Pathog. 2021 Mar 1;17(3):e1009356. doi: 10.1371/journal.ppat.1009356. eCollection 2021 Mar.
8
Screening of Botanical Drugs against Lassa Virus Entry.针对拉沙病毒进入的植物药筛选。
J Virol. 2021 Mar 25;95(8). doi: 10.1128/JVI.02429-20. Epub 2021 Feb 3.
9
Impact of Protein Glycosylation on the Design of Viral Vaccines.蛋白质糖基化对病毒疫苗设计的影响。
Adv Biochem Eng Biotechnol. 2021;175:319-354. doi: 10.1007/10_2020_132.
10
Characterizing the Lassa Virus Envelope Glycoprotein Membrane Proximal External Region for Its Role in Fusogenicity.鉴定拉沙病毒包膜糖蛋白膜近外区在融合性中的作用。
Virol Sin. 2021 Apr;36(2):273-280. doi: 10.1007/s12250-020-00286-3. Epub 2020 Sep 8.