Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China.
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China; Office of Academic Research, Qiqihar Medical University, Qiqihar 161000, China.
Int J Biol Macromol. 2023 Jun 15;240:124414. doi: 10.1016/j.ijbiomac.2023.124414. Epub 2023 Apr 12.
To explore the effect of Lonicera caerulea fruit polyphenols (LCP) on caries-causing bacteria, strain RYX-01 with high production of biofilm and exopolysaccharides (EPS) was isolated from the oral cavity of caries patients and was identified as Lactobacillus rhamnosus by 16S rDNA analysis and morphology. The characteristics of EPS produced by RYX-01 (EPS-CK) and those produced by adding L. caerulea fruit polyphenols (EPS-LCP) were compared to reveal whether LCP reduced the cariogenicity of RYX-01 by influencing the structure and composition of EPS. The results showed that LCP could increase the content of galactose in EPS and destroy the original aggregation state of EPS-CK but had no significant effect on the molecular weight and functional group composition of EPS (p > 0.05). At the same time, LCP could inhibit the growth of RYX-01, reduce EPS and biofilm formation and inhibit the expression of quorum sensing (QS, luxS)- and biofilm formation (wzb)-related genes. Therefore, LCP could change the surface morphology, content and composition of RYX-01 EPS and reduce the cariogenic effect of EPS and biofilm. In conclusion, LCP can be used as a potential plaque biofilm inhibitor and QS inhibitor in drugs and functional foods.
为了探究金银花果实多酚（LCP）对致龋菌的作用，从龋病患者口腔中分离出一株产膜和胞外多糖（EPS）能力较强的菌株 RYX-01，经 16S rDNA 分析和形态学鉴定为鼠李糖乳杆菌。比较 RYX-01 产生的 EPS（EPS-CK）和添加金银花果实多酚产生的 EPS（EPS-LCP）的特征，以揭示 LCP 是否通过影响 EPS 的结构和组成降低 RYX-01 的致龋性。结果表明，LCP 可以增加 EPS 中半乳糖的含量，破坏 EPS-CK 的原有聚集状态，但对 EPS 的分子量和官能团组成没有显著影响（p＞0.05）。同时，LCP 可以抑制 RYX-01 的生长，减少 EPS 和生物膜的形成，并抑制群体感应（QS，luxS）和生物膜形成（wzb）相关基因的表达。因此，LCP 可以改变 RYX-01 EPS 的表面形态、含量和组成，降低 EPS 和生物膜的致龋作用。综上所述，LCP 可作为药物和功能性食品中菌斑生物膜抑制剂和 QS 抑制剂。
