Lebeer Sarah, Verhoeven Tine L A, Perea Vélez Mónica, Vanderleyden Jos, De Keersmaecker Sigrid C J
Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, 3001 Leuven, Belgium.
Appl Environ Microbiol. 2007 Nov;73(21):6768-75. doi: 10.1128/AEM.01393-07. Epub 2007 Sep 7.
Lactobacillus rhamnosus GG (ATCC 53103) is one of the clinically best-studied probiotic organisms. Moreover, L. rhamnosus GG displays very good in vitro adherence to epithelial cells and mucus. Here, we report that L. rhamnosus GG is able to form biofilms on abiotic surfaces, in contrast to other strains of the Lactobacillus casei group tested under the same conditions. Microtiter plate biofilm assays indicated that in vitro biofilm formation by L. rhamnosus GG is strongly modulated by culture medium factors and conditions related to the gastrointestinal environment, including low pH; high osmolarity; and the presence of bile, mucins, and nondigestible polysaccharides. Additionally, phenotypic analysis of mutants affected in exopolysaccharides (wzb), lipoteichoic acid (dltD), and central metabolism (luxS) showed their relative importance in biofilm formation by L. rhamnosus GG.
鼠李糖乳杆菌GG（ATCC 53103）是临床上研究最多的益生菌之一。此外，鼠李糖乳杆菌GG在体外对上皮细胞和黏液表现出很好的黏附性。在此，我们报告，与在相同条件下测试的其他干酪乳杆菌属菌株相比，鼠李糖乳杆菌GG能够在非生物表面形成生物膜。微量滴定板生物膜试验表明，鼠李糖乳杆菌GG的体外生物膜形成受到培养基因素以及与胃肠道环境相关条件的强烈调节，这些条件包括低pH值、高渗透压以及胆汁、黏蛋白和不可消化多糖的存在。此外，对胞外多糖（wzb）、脂磷壁酸（dltD）和中心代谢（luxS）受影响的突变体进行的表型分析显示了它们在鼠李糖乳杆菌GG生物膜形成中的相对重要性。
