Department of Internal Medicine, Akdeniz University School of Medicine, Antalya, Turkey.
Department of Internal Medicine, Division of Nephrology, Akdeniz University School of Medicine, Antalya, Turkey.
Transplant Proc. 2023 Jun;55(5):1140-1146. doi: 10.1016/j.transproceed.2023.03.029. Epub 2023 Apr 13.
This study aims to reveal the relationship between regulatory B cell (Breg) subsets and chronic-active antibody-mediated rejection (c-aABMR) in renal transplant recipients. Our study involved 3 groups of participants: renal transplant recipients with biopsy-proven c-aABMR as the chronic rejection group (c-aABMR, n = 23), recipients with stable graft functions as the patient control group (PC; n = 11), and healthy volunteers (HV; n = 11). Breg subsets, immature/transitional B cells, plasmablastic cells, B10 cells, and BR1 cells were isolated from venous blood samples by flow cytometry. The median values of Breg frequencies in the total lymphocyte population were analyzed. There were no significant differences between the study groups for immature and/or transitional B cell frequencies. Plasmablastic cell frequencies of the c-aABMR group (7.80 [2.10-27.40]) and the PC group (6.00 [1.80-55.50]) were similar, but both of these values were significantly higher than the HVs' (3.40 [1.20-8.50]), (respectively, P = .005 and P = .039). B10 cell frequencies were also similar, comparing the c-aABMR (4.20 [0.10-7.40]) and the PC groups (4.10 [0.10-5.90]), whereas the HVs (5.90 [2.90-8.50]) had the highest B10 cell frequency with an only statistical significance against the PC group (respectively, P = .09 and P = .028). The c-aABMR and the PC groups were similar regarding BR1 cell frequencies. However, the HV group significantly had the highest frequency of BR1 cells (5.50 [2.80-10.80]) than the other groups (P < .001 for both). We demonstrated that frequencies of B10 and BR1 cells were higher in HVs than in transplant recipients, regardless of rejection state. However, there was no significant relation between Breg frequencies and the c-aABMR state.
本研究旨在揭示调节性 B 细胞（Breg）亚群与肾移植受者慢性活动性抗体介导排斥反应（c-aABMR）之间的关系。我们的研究包括三组参与者：经活检证实为 c-aABMR 的肾移植受者为慢性排斥组（c-aABMR，n=23）、移植物功能稳定的受者为患者对照组（PC；n=11）和健康志愿者（HV；n=11）。通过流式细胞术从静脉血样中分离 Breg 亚群、未成熟/过渡性 B 细胞、浆母细胞、B10 细胞和 BR1 细胞。分析总淋巴细胞群体中 Breg 频率的中位数。三组研究对象之间未成熟和/或过渡性 B 细胞频率无显著差异。c-aABMR 组（7.80 [2.10-27.40]）和 PC 组（6.00 [1.80-55.50]）的浆母细胞频率相似，但均显著高于 HVs（3.40 [1.20-8.50]）（分别为 P=0.005 和 P=0.039）。B10 细胞频率也相似，c-aABMR 组（4.20 [0.10-7.40]）和 PC 组（4.10 [0.10-5.90]）相似，而 HVs（5.90 [2.90-8.50]）的 B10 细胞频率最高，与 PC 组相比仅具有统计学意义（分别为 P=0.09 和 P=0.028）。c-aABMR 组和 PC 组的 BR1 细胞频率相似。然而，HV 组的 BR1 细胞频率明显高于其他组（均 P<0.001）。我们表明，无论排斥状态如何，HV 中的 B10 和 BR1 细胞频率均高于移植受者。然而，Breg 频率与 c-aABMR 状态之间无显著关系。
