Bacterial Virulence and Chronic Infections, INSERM U1047, University of Montpellier Department of Microbiology and Hospital Hygiene, University Hospital Nîmes, Nîmes, France.
Université Paris-Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SPI, Bagnols-sur-Cèze, France.
Proteomics Clin Appl. 2023 Jul;17(4):e2200069. doi: 10.1002/prca.202200069. Epub 2023 Apr 27.
Helcococcus kunzii is a skin commensal, Gram-positive bacterium, mostly isolated from infected chronic wounds. This opportunistic pathogen is usually co-isolated with Staphylococcus aureus. The present dataset explores the production and secretion of H. kunzii bacterial virulence interacting proteins in a growth medium mimicking chronic wounds in exponential and stationary growth phases.
The H. kunzii cellular proteome and exoproteome were assessed by analyzing three biological replicates per condition tested. Samples were analyzed using a Q-Exactive HF mass spectrometer. Comparative and functional analyses were performed to profile the identified protein set.
The H. kunzii's cellular proteome encompassed 969 proteins, among which 64 and 53 were specifically identified in the exponential and stationary phase of growth, respectively. Its exoproteome comprised 58 proteins, among which 16 and 14 were characteristic of each growth stage. Metabolic differences between the two phases of growth are discussed. Besides, the production of previously shortlisted and novel putative H. kunzii targets involved in modulating the virulence of S. aureus is investigated.
This work, pioneering the study of H. kunzii physiology in a chronic wound-like environment, should assist future research on this opportunistic pathogen and the search for innovative approaches for wound management.
Helcococcus kunzii 是一种皮肤共生的革兰氏阳性菌，主要从感染的慢性伤口中分离出来。这种机会性病原体通常与金黄色葡萄球菌共同分离。本数据集探索了在模拟慢性伤口的生长培养基中，Helcococcus kunzii 细菌毒力相互作用蛋白的产生和分泌，分别在指数生长期和静止期进行研究。
通过分析每个测试条件的三个生物学重复样本，评估了 H. kunzii 细胞蛋白质组和外蛋白质组。使用 Q-Exactive HF 质谱仪对样品进行分析。进行了比较和功能分析，以对鉴定的蛋白质组进行分析。
H. kunzii 的细胞蛋白质组包含 969 种蛋白质，其中分别有 64 种和 53 种在指数生长期和静止期特异性鉴定。其外蛋白质组包含 58 种蛋白质，其中 16 种和 14 种分别是每个生长阶段的特征。讨论了两个生长阶段之间的代谢差异。此外，还研究了以前有针对性和新型的 H. kunzii 候选物的产生，这些候选物参与调节金黄色葡萄球菌的毒力。
这项在慢性伤口样环境中研究 Helcococcus kunzii 生理学的开创性工作，应该有助于对这种机会性病原体的未来研究和寻找创新的伤口管理方法。
