Qin Liu, Lu Enke, Chen Kexin, Bao Rizhen, Liang Lina, Hu Xiaohu
Key Laboratory for Conservation and Utilization of subtropical Bio-Resources Education Department of Guangxi Zhuang Autonomous Region, Yulin Normal University, Yulin, China.
Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology, Yulin Normal University, Yulin, China.
Mitochondrial DNA B Resour. 2023 Apr 10;8(4):497-500. doi: 10.1080/23802359.2023.2197089. eCollection 2023.
(L.) Kuntze 1891 is a hemiparasitic plant native to Asia and Africa. It is invasive and causes yield losses in crops such as corn, rice and sorghum. Lack of chloroplast genomic data has limited research into its obligate parasitic lifestyle. In this study, the complete chloroplast genome of was sequenced and characterized. It is a quadripartite structure with a total length of 191,085 bp and a GC content of 37.86%. It has a large single copy region (LSC) of 51,406 bp, a small single copy region (SSC) of 273 bp, and two copies of the reverse repeat sequence (IRA and IRB) of 69,703 bp. A total of 122 protein-coding genes, 8 rRNA genes, and 44 tRNA genes were annotated in the chloroplast genome. There were a lot of gene deletions and pseudogenizations in this chloroplast genome. For example, A, D, E, H, I, and K were all pseudogenes because they were missing the 5' end start codon. B, C, and J had shorter gene lengths than their homologs, and F and G were missing genes. The phylogenetic tree reveals that all species form a clade, and a bootstrap value of 100 indicates that is closely related to and . The comprehensive chloroplast genomic resource of would assist researchers in comprehending hemiparasitic mechanisms, molecular markers, and evolutionary patterns of the genus .
(L.) Kuntze 1891是一种原产于亚洲和非洲的半寄生植物。它具有侵入性，会导致玉米、水稻和高粱等作物产量损失。叶绿体基因组数据的缺乏限制了对其专性寄生生活方式的研究。在本研究中，对 的完整叶绿体基因组进行了测序和特征分析。它是一种四分体结构，全长191,085 bp，GC含量为37.86%。它有一个51,406 bp的大单拷贝区域（LSC）、一个273 bp的小单拷贝区域（SSC）以及两个69,703 bp的反向重复序列拷贝（IRA和IRB）。叶绿体基因组中共注释了122个蛋白质编码基因、8个rRNA基因和44个tRNA基因。该叶绿体基因组存在大量基因缺失和假基因化现象。例如，A、D、E、H、I和K均为假基因，因为它们缺少5'端起始密码子。B、C和J的基因长度比其同源基因短，F和G缺失基因。系统发育树显示，所有 物种形成一个分支，自展值为100表明 与 和 密切相关。 的综合叶绿体基因组资源将有助于研究人员理解该属的半寄生机制、分子标记和进化模式。
