Wang Mingcheng, Zhang Shuqiao, Zhang Lei
Chengdu University, Institute for Advanced Study, Chengdu, China.
Engineering Research Center of Sichuan-Tibet Traditional Medicinal Plant, Chengdu, China.
Genet Mol Biol. 2024 Sep 2;47(3):e20240010. doi: 10.1590/1678-4685-GMB-2024-0010. eCollection 2024.
Pedicularis L., a generally bothersome genus of hemiparasitic plants, is primarily native to southwestern China. The phylogenetic relationship and evolutionary history of this genus have not yet been fully resolved. In this study, we sequenced and assembled chloroplast genomes of three Pedicularis species, P. chinensis, P. melampyriflora, and P. striata using high-throughput Illumina sequencing. The assembled plastomes were 142,059 bp (P. chinensis) to 152,146 bp (P. striata) in size, containing 110 (P. chinensis) to 117 (P. striata) genes. Moreover, we identified 13-15 pseudogenes within the three plastomes, nine of which were pseudogenized in all three species. The three plastomes exhibited a similar codon usage pattern. Moreover, the plastomes contained abundant simple sequence repeats and long repeats, which showed slight variations between the three species. A maximum likelihood analysis was performed to elucidate the phylogenetic positions of the three species within the Pedicularis genus. The plastomes presented in our study can be used as valuable genomic resources for further genetic and genomic studies of the Pedicularis genus.
马先蒿属是一类通常令人困扰的半寄生植物，主要原产于中国西南部。该属的系统发育关系和进化历史尚未完全厘清。在本研究中，我们使用高通量Illumina测序对三种马先蒿属植物（华马先蒿、穗花马先蒿和条纹马先蒿）的叶绿体基因组进行了测序和组装。组装后的质体基因组大小为142,059 bp（华马先蒿）至152,146 bp（条纹马先蒿），包含110个（华马先蒿）至117个（条纹马先蒿）基因。此外，我们在这三个质体基因组中鉴定出13至15个假基因，其中9个在所有三个物种中都已假基因化。这三个质体基因组表现出相似的密码子使用模式。此外，质体基因组包含丰富的简单序列重复和长重复序列，这三个物种之间存在轻微差异。进行了最大似然分析以阐明这三个物种在马先蒿属中的系统发育位置。我们研究中呈现的质体基因组可作为有价值的基因组资源，用于马先蒿属进一步的遗传和基因组研究。
