Liang Ze-Wei, Li Si-Yuan, Zhang Xiao-Lin, Chen Chuan-Yue, Sun Wen-Jing, Gu Zhong-Qi, Huang Ji, He Jian-Yu, Qi Peng-Zhi, Guo Bao-Ying, Liao Zhi, Yan Xiao-Jun
Laboratory of Marine Biology Protein Engineering, Marine Science and Technical College, Zhejiang Ocean University, Zhoushan, Zhejiang, China.
College of Marine Sciences, Ningbo University, Ningbo, Zhejiang, China.
Front Physiol. 2023 Mar 30;14:1150521. doi: 10.3389/fphys.2023.1150521. eCollection 2023.
is a dominant shellfish in the Yangtze estuary and its adjacent sea area. Food deprivation often occurs during their growth due to fluctuations in algal abundance caused by seasonal freshwater flushing and high-density aquaculture mode. To investigate the coping strategies of to starvation stress, electron microscopy and differential proteomic analysis were performed on the critical feeding organ gill of the mussels after 9 days of starvation. The electron microscopy results showed that the cilia of the mussel gills were dissolved, and the gaps between gill filaments widened under starvation. Differential proteomic analysis revealed that phagocytosis-related proteins such as ATPeV1E, ATPeV1C, LAMP1_2 and CTSL were significantly upregulated, and the phagocytosis pathway was significantly enriched ( < 0.05). In addition, the corin content in gill and myeloperoxidase level as well as the number of dead cells in blood were both significantly increased ( < 0.05). What's more, proteomic data suggested that immune maintenance, cellular transport and metabolism related pathways were significantly enriched, which illustrated an immune and metabolism responses under starvation. This study reveals for the first time that phagocytosis functions as an essential strategy for to cope with starvation, which provides new scientific knowledge and a theoretical basis for understanding the adaptation mechanisms of mussel to starvation and for rational optimization of mussel culture patterns.
是长江河口及其邻近海域的优势贝类。由于季节性淡水冲刷和高密度养殖模式导致藻类丰度波动，它们在生长过程中经常出现食物匮乏的情况。为了研究应对饥饿胁迫的策略，在饥饿9天后，对贻贝的关键摄食器官鳃进行了电子显微镜和差异蛋白质组分析。电子显微镜结果显示，饥饿条件下贻贝鳃的纤毛溶解，鳃丝间的间隙变宽。差异蛋白质组分析表明，与吞噬作用相关的蛋白质如ATPeV1E、ATPeV1C、LAMP1_2和CTSL显著上调，吞噬作用途径显著富集（<0.05）。此外，鳃中的corin含量、髓过氧化物酶水平以及血液中的死细胞数量均显著增加（<0.05）。更重要的是，蛋白质组数据表明免疫维持、细胞运输和代谢相关途径显著富集，这说明了饥饿条件下的免疫和代谢反应。本研究首次揭示吞噬作用是应对饥饿的重要策略，为理解贻贝对饥饿的适应机制和合理优化贻贝养殖模式提供了新的科学知识和理论基础。
