Azizan Awanis, Venter Leonie, Jansen van Rensburg Peet J, Ericson Jessica A, Ragg Norman L C, Alfaro Andrea C
Aquaculture Biotechnology Research Group, School of Science, Auckland University of Technology, Private Bag 92006, Auckland 1142, New Zealand.
Human Metabolomics, North-West University, Potchefstroom 2520, South Africa.
Metabolites. 2023 Jul 3;13(7):815. doi: 10.3390/metabo13070815.
Temperature is considered to be a major abiotic factor influencing aquatic life. Marine heatwaves are emerging as threats to sustainable shellfish aquaculture, affecting the farming of New Zealand's green-lipped mussel [ (Gmelin, 1791)]. In this study, were gradually exposed to high-temperature stress, mimicking a five-day marine heatwave event, to better understand the effects of heat stress on the metabolome of mussels. Following liquid chromatography-tandem mass spectrometry analyses of haemolymph samples, key sugar-based metabolites supported energy production via the glycolysis pathway and TCA cycle by 24 h and 48 h of heat stress. Anaerobic metabolism also fulfilled the role of energy production. Antioxidant molecules acted within thermally stressed mussels to mitigate oxidative stress. Purine metabolism supported tissue protection and energy replenishment. Pyrimidine metabolism supported the protection of nucleic acids and protein synthesis. Amino acids ensured balanced intracellular osmolality at 24 h and ammonia detoxification at 48 h. Altogether, this work provides evidence that has the potential to adapt to heat stress up to 24 °C by regulating its energy metabolism, balancing nucleotide production, and implementing oxidative stress mechanisms over time. The data reported herein can also be used to evaluate the risks of heatwaves and improve mitigation strategies for aquaculture.
温度被认为是影响水生生物的主要非生物因素。海洋热浪正成为可持续贝类养殖的威胁，影响着新西兰绿唇贻贝[（Gmelin，1791）]的养殖。在本研究中，贻贝被逐渐暴露于高温胁迫下，模拟为期五天的海洋热浪事件，以更好地了解热应激对贻贝代谢组的影响。在对血淋巴样本进行液相色谱-串联质谱分析后，关键的糖基代谢物在热应激24小时和48小时时通过糖酵解途径和三羧酸循环支持能量产生。无氧代谢也起到了能量产生的作用。抗氧化分子在受热应激的贻贝体内发挥作用以减轻氧化应激。嘌呤代谢支持组织保护和能量补充。嘧啶代谢支持核酸保护和蛋白质合成。氨基酸在24小时时确保细胞内渗透压平衡，在48小时时进行氨解毒。总之，这项工作提供了证据，表明贻贝有潜力通过调节其能量代谢、平衡核苷酸产生以及随着时间推移实施氧化应激机制来适应高达24°C的热应激。本文报道的数据还可用于评估热浪风险并改进水产养殖的缓解策略。
