Xiang Xuyu, Peng Bo, Liu Kai, Wang Tianyin, Ding Peng, Li Hao, Zhu Yi, Ming Yingzi
The Transplantation Center of the Third Xiangya Hospital, Central South University, Changsha, China.
Engineering and Technology Research Center for Transplantation Medicine of National Health Commission, Changsha, China.
Front Microbiol. 2023 Mar 29;14:1122101. doi: 10.3389/fmicb.2023.1122101. eCollection 2023.
Renal transplantation is an effective treatment for the end stage renal disease (ESRD). However, how salivary microbiota changes during perioperative period of renal transplant recipients (RTRs) has not been elucidated.
Five healthy controls and 11 RTRs who had good recovery were enrolled. Saliva samples were collected before surgery and at 1, 3, 7, and 14 days after surgery. 16S rRNA gene sequencing was performed.
There was no significant difference in the composition of salivary microbiota between ESRD patients and healthy controls. The salivary microbiota of RTRs showed higher operational taxonomic units (OTUs) amount and greater alpha and beta diversity than those of ESRD patients and healthy controls, but gradually stabilized over time. At the phylum level, the relative abundance of Actinobacteria, Tenericutes and Spirochaetes was about ten times different from ESRD patients or healthy controls for RTRs overall in time. The relative abundance of Bacteroidetes, Fusobacteria, Patescibacteria, Leptotrichiaceae and Streptococcaceae was correlated with serum creatinine (Scr) after renal transplantation.
In short, salivary microbiota community altered in the perioperative period of renal transplantation and certain species of salivary microbiota had the potential to be a biomarker of postoperative recovery.
肾移植是终末期肾病（ESRD）的有效治疗方法。然而，肾移植受者（RTRs）围手术期唾液微生物群如何变化尚未阐明。
招募5名健康对照者和11名恢复良好的RTRs。在手术前以及手术后第1、3、7和14天收集唾液样本。进行16S rRNA基因测序。
ESRD患者和健康对照者唾液微生物群的组成没有显著差异。RTRs的唾液微生物群显示出比ESRD患者和健康对照者更高的操作分类单元（OTUs）数量以及更大的α和β多样性，但随时间逐渐稳定。在门水平上，放线菌门、柔膜菌门和螺旋体门的相对丰度总体上随时间与ESRD患者或健康对照者相比差异约为10倍。拟杆菌门、梭杆菌门、Patescibacteria、纤毛菌科和链球菌科的相对丰度与肾移植后的血清肌酐（Scr）相关。
简而言之，肾移植围手术期唾液微生物群群落发生改变，某些唾液微生物群物种有可能成为术后恢复的生物标志物。
