Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, Taiwan.
Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan.
Front Cell Infect Microbiol. 2022 Apr 26;12:726256. doi: 10.3389/fcimb.2022.726256. eCollection 2022.
Gut microbiota have been targeted by alternative therapies for non-communicable diseases. We examined the gut microbiota of a healthy Taiwanese population, identified various bacterial drivers in different demographics, and compared them with dialysis patients to associate kidney disease progression with changes in gut microbiota.
This was a cross-sectional cohort study.
Fecal samples were obtained from 119 healthy Taiwanese volunteers, and 16S rRNA sequencing was done on the V3-V4 regions to identify the bacterial enterotypes. Twenty-six samples from the above cohort were compared with fecal samples from 22 peritoneal dialysis and 16 hemodialysis patients to identify species-level bacterial biomarkers in the dysbiotic gut of chronic kidney disease (CKD) patients.
Specific bacterial species were identified pertaining to different demographics such as gender, age, BMI, physical activity, and sleeping habits. Dialysis patients had a significant difference in gut microbiome composition compared to healthy controls. The most abundant genus identified in CKD patients was , and at the species level hemodialysis patients showed significant abundance in , , and peritoneal dialysis patients showed higher abundance in (p ≤ 0.05) than the control group. Pathways pertaining to the production of uremic toxins were enriched in CKD patients. The abundance of the bacterial species depended on the type of dialysis treatment.
This study characterizes the healthy gut microbiome of a Taiwanese population in terms of various demographics. In a case-control examination, the results showed the alteration in gut microbiota in CKD patients corresponding to different dialysis treatments. Also, this study identified the bacterial species abundant in CKD patients and their possible role in complicating the patients' condition.
肠道微生物群已成为治疗非传染性疾病的替代疗法的靶点。我们研究了健康台湾人群的肠道微生物群，确定了不同人群中的各种细菌驱动因素，并将其与透析患者进行比较，以将肾脏疾病的进展与肠道微生物群的变化联系起来。
这是一项横断面队列研究。
从 119 名健康台湾志愿者中获得粪便样本，并对 V3-V4 区域进行 16S rRNA 测序，以确定细菌肠型。从上述队列中选择 26 个样本与 22 名腹膜透析和 16 名血液透析患者的粪便样本进行比较，以确定慢性肾脏病（CKD）患者肠道菌群失调中的物种水平细菌生物标志物。
确定了与不同人群（如性别、年龄、BMI、身体活动和睡眠习惯）相关的特定细菌物种。与健康对照组相比，透析患者的肠道微生物组组成存在显著差异。在 CKD 患者中最丰富的属是 ，在物种水平上，血液透析患者中显著丰富的是 、 、腹膜透析患者中显著丰富的是 （p≤0.05）。与 CKD 患者相关的尿毒症毒素产生途径丰富。细菌物种的丰度取决于透析治疗的类型。
本研究从不同人群的角度描述了台湾人口的健康肠道微生物群。在病例对照检查中，结果表明 CKD 患者的肠道微生物群发生了改变，与不同的透析治疗相对应。此外，本研究还确定了丰富存在于 CKD 患者中的细菌物种及其在使患者病情复杂化方面的可能作用。
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