Wang Jiajia, Xiu Lijun, Qiao Ying, Zhang Youyu
Fisheries College, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen, Fujian, China.
Fourth Institute of Oceanography, Key Laboratory of Tropical Marine Ecosystem and Bioresource, Ministry of Natural Resources, Beihai, China.
Front Vet Sci. 2023 Mar 29;10:1172123. doi: 10.3389/fvets.2023.1172123. eCollection 2023.
Psychrophilic could not grow above 25°C and therefore thought unable to infect mammals and humans. In our previous study, a mesophilic SRW-OG1 was isolated from with furunculosis. Through the analysis of preliminary RNA-seq, it was found that the Zn uptake related genes and might be involved in the virulence regulation of SRW-OG1. Therefore, the purpose of this study was to explore the effect of silencing on the virulence regulation of SRW-OG1. The results showed that the growth of the -RNAi, -RNAi, and -RNAi strains was severely restricted under the Fe starvation, but surprisingly there was no significant difference under the Zn restriction. In the absence of Zn and Fe, the expression level of was significantly increased. The motility, biofilm formation, adhesion and hemolysis of the -RNAi, -RNAi, and -RNAi strains were significantly reduced. We also detected the expression of under different growth periods, temperatures, pH, as well as Cu and Pb stresses. The results showed that was significantly up-regulated in the logarithmic phase and the decline phase of . Interestingly, the trend of expression levels of the at 18, 28, and 37°C was reversed to another Zn uptake related gene . Taken together, these indicated that the was necessary for SRW-OG1 pathogenicity and environmental adaptability, and was cross regulated by iron starvation, but it was not irreplaceable for SRW-OG1 Zn uptake in the host.
嗜冷菌无法在25°C以上生长，因此被认为无法感染哺乳动物和人类。在我们之前的研究中，从患有疖疮病的[相关对象未明确]中分离出了一株嗜温菌SRW-OG1。通过初步的RNA测序分析发现，与锌摄取相关的基因[具体基因未明确]可能参与了SRW-OG1的毒力调节。因此，本研究的目的是探讨[相关基因未明确]沉默对SRW-OG1毒力调节的影响。结果表明，[相关基因未明确]-RNAi、[相关基因未明确]-RNAi和[相关基因未明确]-RNAi菌株在铁饥饿条件下生长受到严重限制，但令人惊讶的是，在锌限制条件下没有显著差异。在缺乏锌和铁的情况下，[相关基因未明确]的表达水平显著增加。[相关基因未明确]-RNAi、[相关基因未明确]-RNAi和[相关基因未明确]-RNAi菌株的运动性、生物膜形成、黏附性和溶血能力均显著降低。我们还检测了[相关基因未明确]在不同生长时期、温度、pH以及铜和铅胁迫下的表达情况。结果表明，[相关基因未明确]在[相关菌株未明确]的对数期和衰退期显著上调。有趣的是，在18、28和37°C时，[相关基因未明确]的表达水平趋势与另一个与锌摄取相关的基因[具体基因未明确]相反。综上所述，这些结果表明[相关基因未明确]对SRW-OG1的致病性和环境适应性是必需的，并且受铁饥饿的交叉调节，但对于SRW-OG1在宿主体内摄取锌并非不可替代。
