Fisheries College, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen, China.
Fisheries College, Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Jimei University, Xiamen, China.
Front Immunol. 2021 May 4;12:678699. doi: 10.3389/fimmu.2021.678699. eCollection 2021.
Previously, the dual RNA-seq was carried out in a - infection model to investigate the dynamics of pathogen-host interplay , a member of ZnuCBA Zn importer, was found transcriptionally up-regulated during infection. Thus, this study aimed to assess its role during the trade-off for Zn between host and . ICP-MS analysis and fluorescent staining showed that Zn was withheld from serum and accumulated in the spleen, with increased Zn uptake in the Golgi apparatus of macrophages after infection. Additionally, growth assay, macrophage infection and animal infection after gene knockout / silencing revealed that was necessary for growth in Zn-limiting conditions, colonization, intracellular viability, immune escape and virulence of . Further analysis with dual RNA-seq revealed associations of host's Zn nutritional immunity genes with bacterial Zn assimilation genes. and played key roles in this network, and markedly affected expression, intracellular viability and immune escape, as revealed by gene silencing. Moreover, EMSA and GFP reporter gene analysis showed that Fur sensed changes in Fe concentration to regulate in . Jointly, these findings suggest a trade-off for Zn between host and , while is important for Zn acquisition.
先前，我们在 - 感染模型中进行了双重 RNA-seq 实验，以研究病原体-宿主相互作用的动态。在感染过程中，我们发现一种 ZnuCBA Zn 输入蛋白的成员转录水平上调。因此，本研究旨在评估其在宿主和之间的 Zn 权衡中所起的作用。ICP-MS 分析和荧光染色显示，Zn 被阻止从血清中进入，并在感染后积累在脾脏中，巨噬细胞高尔基体中的 Zn 摄取增加。此外，生长测定、巨噬细胞感染和基因敲除/沉默后的动物感染表明，在 Zn 限制条件下的生长、定植、细胞内活力、免疫逃避和毒力都需要 。进一步的双重 RNA-seq 分析显示宿主的 Zn 营养免疫基因与细菌 Zn 同化基因之间存在关联。和 在这个网络中起着关键作用，并且通过基因沉默显著影响 的表达、细胞内活力和免疫逃避。此外，EMSA 和 GFP 报告基因分析表明，Fur 感应 Fe 浓度的变化来调节 在 中的表达。综上所述，这些发现表明宿主和之间存在 Zn 权衡，而 对于 的 Zn 获得很重要。
