Levin Aleksandr A, Karalkin Pavel A, Koudan Elizaveta V, Senatov Fedor S, Parfenov Vladislav A, Lvov Vladislav A, Petrov Stanislav V, Pereira Frederico D A S, Kovalev Alexey V, Osidak Egor O, Domogatsky Sergey P, Manturova Natalya E, Kasyanov Vladimir A, Sergeeva Natalia S, Zorin Vadim L, Khesuani Yusef D, Mironov Vladimir A
Center for Biomedical Engineering, National University of Science and Technology "MISIS", Moscow, Russia.
National Medical Research Radiological Center, P. A. Hertsen Moscow Oncology Research Center, Moscow, Russia.
Int J Bioprint. 2023 Jan 31;9(2):675. doi: 10.18063/ijb.v9i2.675. eCollection 2023.
bioprinting is one of the most clinically relevant techniques in the emerging bioprinting technology because it could be performed directly on the human body in the operating room and it does not require bioreactors for post-printing tissue maturation. However, commercial bioprinters are still not available on the market. In this study, we demonstrated the benefit of the originally developed first commercial articulated collaborative bioprinter for the treatment of full-thickness wounds in rat and porcine models. We used an articulated and collaborative robotic arm from company KUKA and developed original printhead and correspondence software enabling bioprinting on curve and moving surfaces. The results of and experiments show that bioprinting of bioink induces a strong hydrogel adhesion and enables printing on curved surfaces of wet tissues with a high level of fidelity. The bioprinter was convenient to use in the operating room. Additional experiments ( collagen contraction assay and 3D angiogenesis assay) and histological analyses demonstrated that bioprinting improves the quality of wound healing in rat and porcine skin wounds. The absence of interference with the normal process of wound healing and even certain improvement in the dynamics of this process strongly suggests that bioprinting could be used as a novel therapeutic modality in wound healing.
生物打印是新兴生物打印技术中与临床最为相关的技术之一，因为它可以在手术室直接应用于人体，且无需生物反应器来促进打印后组织的成熟。然而，市面上仍未出现商用生物打印机。在本研究中，我们展示了最初研发的首款商用关节协作式生物打印机在大鼠和猪模型全层伤口治疗中的优势。我们使用了库卡公司的关节协作式机器人手臂，并开发了原创的打印头和配套软件，实现了在曲线和移动表面上进行生物打印。实验结果表明，生物墨水的生物打印能产生强大的水凝胶附着力，并能在湿组织的曲面上以高保真度进行打印。这款生物打印机在手术室使用很方便。额外的实验（胶原蛋白收缩试验和三维血管生成试验）及组织学分析表明，生物打印可改善大鼠和猪皮肤伤口的愈合质量。生物打印对伤口愈合正常过程无干扰，甚至在该过程的动态变化方面有一定改善，这有力地表明生物打印可作为伤口愈合的一种新型治疗方式。
