Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
J Tissue Eng Regen Med. 2021 Jun;15(6):546-555. doi: 10.1002/term.3194. Epub 2021 Apr 5.
Three dimensional (3D) printing has recently expanded in popularity and has become an effective approach for tissue engineering. Advances in tissue engineering have increased the effectiveness of cell-based therapies. Indeed, the ultimate goal of such treatment is the development of conditions similar to fetal wound regeneration. In this context, technology of 3D printing also allows researchers to more effectively compose multi-material and cell-laden scaffolds with less effort. In this study, we explored a synthetic gel scaffold derived from 3D bioprinter with or without stem cells to accelerate wound healing and skin defects. Adipose-derived stem cells (ADSCs) were isolated and seeded into 3D bioprinter derived-gel scaffold. Morphological and cell adherence properties of 3D scaffold were assessed by hemotoxylin & eosin (H&E) staining and scanning electron microscopy and cell viability was determined by methylthiazolyldiphenyl-tetrazolium bromide assay. In vivo assessment of the scaffold was done using H&E staining in the full-thickness burn rat model. The experimental groups included; (a) untreated (control), (b) 3D bioprinter derived-gel scaffold (Trial 1), and (c) 3D bioprinter derived-gel scaffold loaded with ADSC (Trial 2). Our results represented 3D bioprinter derived-gel scaffold with or without ADSCs accelerated wound contraction and healing compared to control groups. Epithelization was completed until 21 days after operation in scaffold alone. In scaffold with ADSCs group, epithelization was faster and formed a multi-layered epidermis with the onset of cornification. In conclusion, 3D bioprinter derived-gel scaffold with or without ADSCs has the potential to be used as a wound graft material in skin regenerative medicine.
三维（3D）打印技术最近越来越受欢迎，已成为组织工程的有效方法。组织工程学的进步提高了基于细胞的治疗方法的效果。实际上，这种治疗的最终目标是开发类似于胎儿伤口再生的条件。在这种情况下，3D 打印技术还使研究人员能够更有效地用更少的精力组成多材料和细胞负载支架。在这项研究中，我们探索了一种源自 3D 生物打印机的合成凝胶支架，该支架带有或不带有干细胞，以加速伤口愈合和皮肤缺损。分离并接种脂肪来源的干细胞（ADSCs）到 3D 生物打印机衍生的凝胶支架中。通过苏木精和曙红（H＆E）染色和扫描电子显微镜评估 3D 支架的形态和细胞黏附特性，并通过甲基噻唑基四唑溴化物测定法确定细胞活力。通过 H＆E 染色在全厚度烧伤大鼠模型中进行支架的体内评估。实验组包括：（a）未处理（对照），（b）3D 生物打印机衍生的凝胶支架（试验 1）和（c）负载 ADSC 的 3D 生物打印机衍生的凝胶支架（试验 2）。我们的结果代表了具有或不具有 ADSCs 的 3D 生物打印机衍生的凝胶支架可加速伤口收缩和愈合，与对照组相比。单独使用支架时，上皮化在手术后 21 天完成。在载有 ADSCs 的支架组中，上皮化更快，并在角蛋白形成开始时形成多层表皮。总之，具有或不具有 ADSCs 的 3D 生物打印机衍生的凝胶支架具有作为皮肤再生医学中伤口移植物材料的潜力。
