Guo Zhichao, Liu Yilin, Wang Chengjuan, Li Shujun, Yu Lei, Wu Wenzhi, You Xu, Zhang Yunqiao, Teng Zhaowei, Zeng Yong
The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
The Third Affiliated Hospital of Kunming Medical University, Kunming, China.
Front Psychiatry. 2023 Mar 31;14:1126615. doi: 10.3389/fpsyt.2023.1126615. eCollection 2023.
Non-suicidal self-injury (NSSI) is self-injurious behavior without suicidal intent commonly seen in the adolescent population and poses a serious threat to the life safety of adolescents. Related researches suggest a possible correlation between addiction and the occurrence of NSSI. This study aimed to explore the correlation between addiction and NSSI from a molecular biological perspective by analyzing the differential expression of addiction-related genes in NSSI patients.
(1) The association between addiction and non-suicidal self-injury in a Chinese adolescent population was verified with the help of questionnaires on substance and non-substance addictions and non-suicidal self-injury among 1,329 adolescents in China, (2) Screening for key genes associated with addiction by bioinformatics analysis, and (3) RT-qPCR experiment was performed to validate key genes and Receiver Operating Characteristic curves were plotted for target genes.
(1) Substance and non-substance addictions were all significantly correlated with non-suicidal self-injury, (2) Four target genes: , , and were screened by bioinformatics technique, and (3) Relative quantitative analysis by RT-qPCR revealed that the expression levels of ( < 0.01), ( < 0.05) and ( < 0.01) were significantly higher in NSSI patients than in healthy controls.
(1) The significant association between addiction and NSSI exists in the Chinese adolescent population and (2) Addiction-related genes , , and are differentially expressed in adolescents with NSSI. The genes have the potential to become biological markers for the diagnosis of NSSI.
非自杀性自伤（NSSI）是一种无自杀意图的自伤行为，常见于青少年群体，对青少年的生命安全构成严重威胁。相关研究表明成瘾与NSSI的发生之间可能存在关联。本研究旨在通过分析NSSI患者中成瘾相关基因的差异表达，从分子生物学角度探讨成瘾与NSSI之间的相关性。
（1）借助关于物质和非物质成瘾以及非自杀性自伤的问卷，对1329名中国青少年进行调查，以验证中国青少年群体中成瘾与非自杀性自伤之间的关联；（2）通过生物信息学分析筛选与成瘾相关的关键基因；（3）进行RT-qPCR实验以验证关键基因，并绘制目标基因的受试者工作特征曲线。
（1）物质和非物质成瘾均与非自杀性自伤显著相关；（2）通过生物信息学技术筛选出四个目标基因： 、 、 和 ；（3）RT-qPCR相对定量分析显示，NSSI患者中 （ ＜0.01）、 （ ＜0.05）和 （ ＜0.01）的表达水平显著高于健康对照组。
（1）中国青少年群体中存在成瘾与NSSI之间的显著关联；（2）成瘾相关基因 、 和 在NSSI青少年中差异表达。这些基因有潜力成为诊断NSSI的生物标志物。
