Itoh Y H, Itoh T, Kaneko H
Anal Biochem. 1986 Apr;154(1):200-4. doi: 10.1016/0003-2697(86)90515-4.
An improved microscale method for determining lipid phosphorus was developed. Without the prior removal of nonlipid contaminants from crude lipid extracts, the absolute amount of lipid phosphorus and distribution of phospholipids were directly determined by a two-dimensional thin-layer chromatography-internal standard system, using pyrophosphatidic acid as the internal standard. Application of this method to the assay of lipid phosphorus present in small amounts in brain tissues is discussed.
开发了一种改进的微量测定脂质磷的方法。无需事先从粗脂质提取物中去除非脂质污染物，使用焦磷酸磷脂酸作为内标，通过二维薄层色谱-内标系统直接测定脂质磷的绝对量和磷脂分布。讨论了该方法在脑组织中少量存在的脂质磷测定中的应用。
