一种用于测定缩醛磷脂的二维薄层色谱法。
A two-dimensional thin-layer chromatographic procedure for the estimation of plasmalogens.
作者信息
Owens K
出版信息
Biochem J. 1966 Aug;100(2):354-61. doi: 10.1042/bj1000354.
Abstract
- The use of two-dimensional thin-layer chromatography is described that allows the rapid and simultaneous determination of phospholipid classes and their constituent plasmalogens. 2. The method is based on the specific hydrolysis of plasmalogens to (2-acyl) lysophospholipid in the presence of a mercuric chloride spray reagent. 3. The proportion of mercuric chloride-labile phospholipid present in each phospholipid class, calculated on the basis of phosphorus recoveries from the charred chromatogram, was compared with the proportion of long-chain aldehyde and of total lipid phosphorus found in small-scale preparations of each class of phospholipid. 4. The method permits the determination of individual plasmalogens on preparations containing as little as 0.2mug.atom of total lipid phosphorus.
摘要
- 描述了二维薄层色谱法的应用，该方法可快速同时测定磷脂类别及其组成的缩醛磷脂。2. 该方法基于在氯化汞喷雾试剂存在下，缩醛磷脂特异性水解为（2-酰基）溶血磷脂。3. 根据从烧焦的色谱图中回收的磷，计算出各磷脂类别中对氯化汞不稳定的磷脂比例，并与各类磷脂小规模制备物中长链醛和总脂质磷的比例进行比较。4. 该方法可用于测定总脂质磷含量低至0.2微克原子的制剂中的单个缩醛磷脂。
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