Cardiovascular Research Institute for Intractable Disease, College of Medicine, The Catholic University of Korea.
Cardiovascular Research Institute for Intractable Disease, College of Medicine, The Catholic University of Korea;
J Vis Exp. 2023 Mar 31(193). doi: 10.3791/65078.
Animal models are emerging as an important tool to understand the pathologic mechanisms underlying aortic valve stenosis (AVS) because of the lack of access to reliable sources of diseased human aortic valves. Among the various animal models, AVS rabbit models are one of the most commonly used in large animal studies. However, traditional AVS rabbit models require a long-term period of dietary supplementation and genetic manipulation to induce significant stenosis in the aortic valve, limiting their use in experimental studies. To address these limitations, a new AVS rabbit model is proposed, in which stenosis is induced by a direct balloon injury to the aortic valve. The present protocol describes a successful technique for inducing AVS in New Zealand white (NZW) rabbits, with step-by-step procedures for the preparation, the surgical procedure, and the post-operative care. This simple and reproducible model offers a promising approach for studying the initiation and progression of AVS and provides a valuable tool for investigating the underlying pathological mechanisms of the disease.
动物模型作为一种重要的工具，在缺乏可靠的人类主动脉瓣疾病来源的情况下，用于研究主动脉瓣狭窄（AVS）的病理机制。在各种动物模型中，AVS 兔模型是大型动物研究中最常用的模型之一。然而，传统的 AVS 兔模型需要长期的饮食补充和基因操作来诱导主动脉瓣的显著狭窄，限制了它们在实验研究中的应用。为了解决这些限制，提出了一种新的 AVS 兔模型，其中通过直接球囊损伤主动脉瓣来诱导狭窄。本方案描述了一种在新西兰白兔（NZW）中成功诱导 AVS 的技术，包括准备、手术步骤和术后护理的详细过程。这种简单且可重复的模型为研究 AVS 的起始和进展提供了一种有前途的方法，并为研究疾病的潜在病理机制提供了有价值的工具。
