Center for Low-temperature Plasma Sciences, Nagoya University, Nagoya, Japan.
Department of Obstetrics and Gynecology, Nagoya University, Graduate School of Medicine, Nagoya, Japan.
Free Radic Res. 2023 Feb;57(2):91-104. doi: 10.1080/10715762.2023.2201390. Epub 2023 Apr 17.
Cold atmospheric pressure plasmas are promising medical tools that can assist in cancer treatment. While the medical pathology mechanism is substantially understood, knowledge of the contribution of reactive species formed in plasma and the mode of activation of biochemical pathways is insufficient. Herein, we present a concept involving antitumoral plasma-activated organics, which is envisaged to increase cytotoxicity levels against cancer cells. Ringer's acetate solution was irradiated by low-temperature plasma at atmospheric pressure and possible reaction pathways of the compound generation are presented. The chemical compounds formed by plasma treatment and their effects on non-tumorigenic breast epithelial cells (MCF-10A) and breast cancer cells (MCF-7) were investigated. The cell viability results have shown that plasma-derived compounds have both, stimulatory and inhibitory effects on cell viability, depending on the concentration of the generated compounds in the irradiated liquids. Previous studies have shown that oxidative stresses involving reactive oxygen and nitrogen species (RONS) can be used to kill cancer cells. Hence, while RONS offers promising first-step killing effects, cell viability results have shown that plasma-derived compounds, such as acetic anhydride and ethyl acetate, have the potential to play important roles in plasma-based cancer therapy.
冷等离体大气压等离子体是一种很有前途的医疗工具，可以辅助癌症治疗。虽然其医学病理学机制已得到充分理解，但对于等离子体中形成的反应性物质的贡献以及生物化学途径的激活模式的了解还不够。在此，我们提出了一个涉及抗肿瘤等离子体激活有机物的概念，预计它可以提高对癌细胞的细胞毒性水平。我们用低温等离体大气压等离子体辐照醋酸林格氏溶液，并提出了化合物生成的可能反应途径。研究了等离子体处理形成的化学化合物及其对非致瘤性乳腺上皮细胞（MCF-10A）和乳腺癌细胞（MCF-7）的影响。细胞活力结果表明，等离子体衍生的化合物对细胞活力具有刺激和抑制作用，这取决于辐照液体中生成化合物的浓度。先前的研究表明，涉及活性氧和氮物种（RONS）的氧化应激可以用于杀死癌细胞。因此，虽然 RONS 提供了有希望的第一步杀伤效果，但细胞活力结果表明，等离子体衍生的化合物，如醋酸酐和乙酸乙酯，有可能在基于等离子体的癌症治疗中发挥重要作用。
