College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Henan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Zhengzhou 450046, China.
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Henan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Zhengzhou 450046, China; The Shennong Laboratory, Zhengzhou 45000b, China.
Poult Sci. 2023 Jun;102(6):102665. doi: 10.1016/j.psj.2023.102665. Epub 2023 Mar 17.
Columbian coloration patterns in plumage are widespread phenomena in several standard breeds of poultry, such as the Columbian Plymouth Rock chicken. H line chicken plumage is generally a pure white except in the hackle, wing, and tail plumage, which coloration is very similar to the Columbian plumage pattern, but with the barring substituting for the black vertical striping. Thus, we refer to this plumage coloration as "sub-Columbian" pattern. However, the genetic basis of this phenotype remains unknown. Here, a F3 cross population between yellow plumage roosters and sub-Columbian plumage hens was constructed, for verifying sub-Columbian plumage was sex-linked dominant inheritance. To identify the candidate regions, F2 generation sub-Columbian plumage hens and yellow plumage hens with their parental lines were used for BSA-seq, and sub-Columbian plumage genes were mapped to a 10.46 Mb interval on chromosome Z. Remarkably, by transcriptome analysis of the neck and wing tip follicle tissues of the 2 plumage colors, we demonstrated that within the interval, only 1 gene, SLC45A2 expressed significant differently (P < 0.05). Through KASP, we identified L347M and A10331272T in solute carrier family 45 member 2 (SLC45A2), and B2 haplotype of cyclin-dependent kinase inhibitor 2A (CDKN2A), showed near complete association with the phenotype. Eventually, we designed a hybridization experiment for verifying the locus of sub-Columbian plumage, which is inherited through Z-linked dominant inheritance and is controlled by SLC45A2 and CDKN2A.
禽类羽毛中的哥伦比亚色型是几种标准家禽品种（如哥伦比亚普利茅斯洛克鸡）中广泛存在的现象。H 线鸡的羽毛通常为纯白色，除了颈羽、翼羽和尾羽的颜色与哥伦比亚色型非常相似，但斑纹代替了黑色的垂直条纹。因此，我们将这种羽毛颜色称为“亚哥伦比亚”色型。然而，这种表型的遗传基础尚不清楚。在这里，我们构建了一个黄羽公鸡和亚哥伦比亚色母鸡的 F3 杂交群体，以验证亚哥伦比亚色羽是性连锁显性遗传。为了确定候选区域，我们使用 F2 代的亚哥伦比亚色母鸡和黄羽母鸡及其亲本系进行 BSA-seq 分析，将亚哥伦比亚色基因定位到 Z 染色体上的 10.46Mb 区间。值得注意的是，通过对 2 种羽毛颜色的颈部和翅尖毛囊组织的转录组分析，我们证明在该区间内，只有 1 个基因 SLC45A2 表达显著不同（P<0.05）。通过 KASP，我们在溶质载体家族 45 成员 2（SLC45A2）中鉴定出 L347M 和 A10331272T，以及细胞周期蛋白依赖性激酶抑制剂 2A（CDKN2A）的 B2 单倍型，与表型表现出近乎完全的关联。最终，我们设计了一个杂交实验来验证亚哥伦比亚色羽的基因座，该基因座通过 Z 连锁显性遗传传递，由 SLC45A2 和 CDKN2A 控制。
