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黄喉貂(Martes flavigula)染色体水平基因组组装。

A chromosome-level genome assembly of the yellow-throated marten (Martes flavigula).

机构信息

School of Life Sciences, Qufu Normal University, Qufu, 273165, Shandong, China.

出版信息

Sci Data. 2023 Apr 17;10(1):216. doi: 10.1038/s41597-023-02120-3.

Abstract

The yellow-throated marten (Martes flavigula) is a medium-sized carnivore that is widely distributed across much of Asia and occupies an extensive variety of habitats. We reported a high-quality genome assembly of this organism that was generated using Oxford Nanopore and Hi-C technologies. The final genome sequences contained 215 contigs with a total size of 2,449.15 Mb and a contig N50 length of 68.60 Mb. Using Hi-C analysis, 2,419.20 Mb (98.78%) of the assembled sequences were anchored onto 21 linkage groups. Merqury evaluation suggested that the genome was 94.95% complete with a QV value of 43.75. Additionally, the genome was found to comprise approximately 39.74% repeat sequences, of which long interspersed elements (LINE) that accounted for 26.13% of the entire genome, were the most abundant. Of the 20,464 protein-coding genes, prediction and functional annotation was successfully performed for 20,322 (99.31%) genes. The high-quality, chromosome-level genome of the marten reported in this study will serve as a reference for future studies on genetic diversity, evolution, and conservation biology.

摘要

黄喉貂(Martes flavigula)是一种中型食肉动物,广泛分布于亚洲大部分地区,栖息于多种不同的生境中。我们报道了一种高质量的基因组组装,该基因组是使用牛津纳米孔和 Hi-C 技术生成的。最终的基因组序列包含 215 个contigs,总大小为 2449.15 Mb,contig N50 长度为 68.60 Mb。使用 Hi-C 分析,组装序列的 2419.20 Mb(98.78%)被锚定到 21 个连锁群上。Merqury 评估表明,基因组的完整性为 94.95%,QV 值为 43.75。此外,该基因组大约包含 39.74%的重复序列,其中长散在元件(LINE)占整个基因组的 26.13%,最为丰富。在 20464 个蛋白质编码基因中,成功预测和注释了 20322 个(99.31%)基因。本研究报道的黄喉貂高质量的染色体水平基因组将为未来的遗传多样性、进化和保护生物学研究提供参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50af/10110515/02d2537a9576/41597_2023_2120_Fig1_HTML.jpg

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