文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

酵母细胞壁产物对脂多糖刺激后猪肠道上皮细胞屏障功能恢复的不同影响。

Differential impact of yeast cell wall products in recovery of porcine intestinal epithelial cell barrier function following Lipopolysaccharide challenge.

作者信息

Browne Niall, Daly Daniel, Horgan Karina

机构信息

Alltech Bioscience Centre, Summerhill Road, Dunboyne, Co. Meath, Ireland.

出版信息

Porcine Health Manag. 2023 Apr 17;9(1):18. doi: 10.1186/s40813-023-00312-2.

DOI:10.1186/s40813-023-00312-2
PMID:37069650
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10111678/
Abstract

BACKGROUND: In swine intestinal barrier deterioration can be caused by exposure to harmful bacteria, toxins or contaminants that can lead to a leaky gut and post weaning diarrhoea. A leaky gut leads to increased infection, inflammation and poor nutrient absorption that can impair piglet growth and ultimately survival. Application of yeast cell wall (YCW) products may offer an opportunity to reduce the intestinal barrier damage caused by microbial challenge. A Mannan rich fraction (MRF) and three YCW products were compared by examining their impact on intestinal barrier function using a Jejunal model of intestine in response to a bacterial challenge using Salmonella LPS. RESULTS: Trans epithelial electrical resistance (TEER) readings showed MRF had a significantly higher barrier function (P ≤ 0.05) over the positive control while YCW products A, B and C demonstrated no significant improvement to the positive control. Transcriptome analysis of the IPEC-J2 cells showed that differentially expressed genes associated with the gene ontology (GO) term for Structural molecule activity was significantly upregulated in the MRF treated cells over the positive control cells with 56 genes upregulated compared to product B (50 genes), Product C, (25 genes) and the negative control's 60 genes. Product A had no functional grouping under the structural molecule activity term. Both qPCR and western blotting analysis of tight junction associated genes showed that MRF treated cells demonstrated significantly higher Claudin 3 junctional gene expression (P ≤ 0.05) over the positive control and treatments A, B and C. Occludin expression was significantly higher in MRF treated cells (P ≤ 0.05) over the positive control and product B. A nonsignificant rise in TJP-1 gene expression was observed in the MRF treated cells when compared to the positive control. Protein abundances of Claudin 3, Occludin and TJP-1 were significantly (P ≤ 0.05) higher following MRF application to LPS challenged IPEC-J2 cells over the positive control. CONCLUSIONS: The difference in each YCW products production and composition appeared to influence intestinal barrier integrity. The action of MRF demonstrates its potential ability to raise intestinal barrier integrity of IPEC-J2 intestinal cells on an in vitro level through significantly elevated intracellular connections.

摘要

背景：在猪中，肠道屏障的恶化可能由接触有害细菌、毒素或污染物引起，这会导致肠道渗漏和断奶后腹泻。肠道渗漏会导致感染增加、炎症加剧和营养吸收不良，从而影响仔猪生长并最终影响其存活。应用酵母细胞壁（YCW）产品可能为减少微生物挑战引起的肠道屏障损伤提供机会。通过使用空肠模型，研究甘露聚糖富集部分（MRF）和三种YCW产品对肠道屏障功能的影响，该模型用于应对沙门氏菌脂多糖（Salmonella LPS）的细菌挑战。 结果：跨上皮电阻（TEER）读数显示，与阳性对照相比，MRF具有显著更高的屏障功能（P≤0.05），而YCW产品A、B和C与阳性对照相比未显示出显著改善。对IPEC-J2细胞的转录组分析表明，与结构分子活性的基因本体（GO）术语相关的差异表达基因在MRF处理的细胞中比阳性对照细胞显著上调，与产品B（50个基因）、产品C（25个基因）和阴性对照的60个基因相比，有56个基因上调。产品A在结构分子活性术语下没有功能分组。紧密连接相关基因的qPCR和蛋白质印迹分析均表明，与阳性对照以及处理A、B和C相比，MRF处理的细胞中Claudin 3连接基因表达显著更高（P≤0.05）。与阳性对照和产品B相比，MRF处理的细胞中闭合蛋白（Occludin）表达显著更高（P≤0.05）。与阳性对照相比，MRF处理的细胞中TJP-1基因表达有不显著的升高。将MRF应用于LPS刺激的IPEC-J2细胞后，Claudin 3、Occludin和TJP-1的蛋白质丰度显著高于阳性对照（P≤0.05）。 结论：每种YCW产品的生产和组成差异似乎会影响肠道屏障的完整性。MRF的作用表明其具有通过显著增强细胞内连接在体外提高IPEC-J2肠道细胞肠道屏障完整性的潜在能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6fa/10111678/7b4022d8ad03/40813_2023_312_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6fa/10111678/13dcd02a164c/40813_2023_312_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6fa/10111678/b1b539184a49/40813_2023_312_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6fa/10111678/c0c08907cc92/40813_2023_312_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6fa/10111678/03574b7a17ad/40813_2023_312_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6fa/10111678/60dda62f6bf5/40813_2023_312_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6fa/10111678/7b4022d8ad03/40813_2023_312_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6fa/10111678/13dcd02a164c/40813_2023_312_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6fa/10111678/b1b539184a49/40813_2023_312_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6fa/10111678/c0c08907cc92/40813_2023_312_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6fa/10111678/03574b7a17ad/40813_2023_312_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6fa/10111678/60dda62f6bf5/40813_2023_312_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6fa/10111678/7b4022d8ad03/40813_2023_312_Fig6_HTML.jpg

相似文献

[1]
Differential impact of yeast cell wall products in recovery of porcine intestinal epithelial cell barrier function following Lipopolysaccharide challenge.

Porcine Health Manag. 2023-4-17

[2]
4-Phenylbutyric acid accelerates rehabilitation of barrier function in IPEC-J2 cell monolayer model.

Anim Nutr. 2021-12

[3]
Clostridium Tyrobutyricum Protect Intestinal Barrier Function from LPS-Induced Apoptosis via P38/JNK Signaling Pathway in IPEC-J2 Cells.

Cell Physiol Biochem. 2018

[4]
Short-chain fatty acids modulate the IPEC-J2 cell response to pathogenic E. coli LPS-activated PBMC.

Res Vet Sci. 2024-5

[5]
Eleutheroside B increase tight junction proteins and anti-inflammatory cytokines expression in intestinal porcine jejunum epithelial cells (IPEC-J2).

J Anim Physiol Anim Nutr (Berl). 2019-4-16

[6]
Effects of different short-chain fatty acids (SCFA) on gene expression of proteins involved in barrier function in IPEC-J2.

Porcine Health Manag. 2022-5-19

[7]
Dietary supplementation of weaned piglets with a yeast-derived mannan-rich fraction modulates cecal microbial profiles, jejunal morphology and gene expression.

Animal. 2019-1-7

[8]
Acetate and propionate effects in response to LPS in a porcine intestinal co-culture model.

Porcine Health Manag. 2023-5-23

[9]
Glucagon-like peptide 2 attenuates intestinal mucosal barrier injury through the MLCK/pMLC signaling pathway in a piglet model.

J Cell Physiol. 2021-4

[10]
Protective effects of Lactobacillus plantarum on epithelial barrier disruption caused by enterotoxigenic Escherichia coli in intestinal porcine epithelial cells.

Vet Immunol Immunopathol. 2016-4

引用本文的文献

[1]
Exploring the Interplay between the Hologenome and Complex Traits in Bovine and Porcine Animals Using Genome-Wide Association Analysis.

Int J Mol Sci. 2024-6-5

本文引用的文献

[1]
Weaning Induced Gut Dysfunction and Nutritional Interventions in Nursery Pigs: A Partial Review.

Animals (Basel). 2021-4-29

[2]
Dietary supplementation of weaned piglets with a yeast-derived mannan-rich fraction modulates cecal microbial profiles, jejunal morphology and gene expression.

Animal. 2019-1-7

[3]
Orphan receptor NR4A3 is a novel target of p53 that contributes to apoptosis.

Oncogene. 2018-11-19

[4]
Mechanisms regulating intestinal barrier integrity and its pathological implications.

Exp Mol Med. 2018-8-16

[5]
Weaning stress and gastrointestinal barrier development: Implications for lifelong gut health in pigs.

Anim Nutr. 2017-12

[6]
Airway Epithelial Repair by a Prebiotic Mannan Derived from .

J Immunol Res. 2017-7-9

[7]
The Novel Type 1 Fimbriae FimH Receptor Calreticulin Plays a Role in Host Specificity.

Front Cell Infect Microbiol. 2017-7-19

[8]
Early weaning stress induces chronic functional diarrhea, intestinal barrier defects, and increased mast cell activity in a porcine model of early life adversity.

Neurogastroenterol Motil. 2017-6-1

[9]
Enteric Pathogens and Their Toxin-Induced Disruption of the Intestinal Barrier through Alteration of Tight Junctions in Chickens.

Toxins (Basel). 2017-2-10

[10]
Early life adversity in piglets induces long-term upregulation of the enteric cholinergic nervous system and heightened, sex-specific secretomotor neuron responses.

Neurogastroenterol Motil. 2016-9

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索