Mokhtari Fedra, Kaboosi Hami, Mohebbi Seyed Reza, Asadzadeh Aghdaei Hamid, Zali Mohammad Reza
Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad university, Amol, Iran.
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gastroenterol Hepatol Bed Bench. 2023;16(1):520-523.
Development of an amplification method for further investigation of HBV S gene variation patterns.
Pre-S/S variants in patients with chronic HBV infection may contribute to the progression of liver damage and Hepatocellular carcinoma (HCC).
This study was performed on ten patients with chronic HBV infection. Viral DNA was extracted from patient's plasma, primer design was performed, and a semi-nested PCR method was set up to amplify the pre-S/S region of HBV genome. Subsequently, sequencing was performed to analyze the variants of this region.
In the current study, the semi-nested PCR method was successfully set up, and types of variation in the studied samples were investigated.
Pre-S/S variants should be routinely determined in HBV carriers to help identify individuals who may be at a high risk of less favorable liver disease progression. This study showed that the technique could accurately amplify the pre-S/S region, and the product can be successfully used for variation detection by direct sequencing.
开发一种扩增方法以进一步研究乙肝病毒S基因变异模式。
慢性乙肝病毒感染患者的前S/S变异可能促使肝损伤和肝细胞癌（HCC）进展。
本研究针对10例慢性乙肝病毒感染患者开展。从患者血浆中提取病毒DNA，进行引物设计，并建立半巢式PCR方法以扩增乙肝病毒基因组的前S/S区域。随后进行测序以分析该区域的变异。
在本研究中，成功建立了半巢式PCR方法，并对研究样本的变异类型进行了调查。
应常规检测乙肝病毒携带者的前S/S变异，以帮助识别可能处于肝病进展不利高风险的个体。本研究表明，该技术可准确扩增前S/S区域，其产物可通过直接测序成功用于变异检测。
