Collaborative Innovation Center for Child Nutrition and Health Development, Chongqing Engineering Research Center of Functional Food, Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing, P.R. China.
Department of Gasgtroenterologu, The People's Hospital of Jiulongpo Chongqing, Chongqing, P.R. China.
J Food Sci. 2023 Jun;88(6):2642-2654. doi: 10.1111/1750-3841.16560. Epub 2023 Apr 17.
To decrease the climbing rate of alcoholic liver disease, the protective effect in subacute alcoholic liver injury of newly isolated Lactiplantibacillus pentosus CQZC01 has been investigated. Lactiplantibacillus pentosus CQZC01 (1 × 109 CFU/kgbw) administered orally could keep weight of mice at 30.54 ± 1.15 g; alleviate alcoholic damage on hepatic morphology; decrease the activities of hyaluronidase (147 ± 19 U/L), procollagen III (4.82 ± 0.54 ng/mL), alanine transaminase (10.66 ± 2.32 U/L), and aspartate aminotransferase (15.18 ± 1.98 U/L); enhance the activities of alcohol dehydrogenase (65.15 ± 3.2 U/mgprot), aldehyde dehydrogenase (16.50 ± 0.96 U/mgprot), superoxide dismutase (623 ± 39 U/mgprot), and glutathione (19.54 ± 2.46 µmol/gprot); and decrease liver total cholesterol (3.59 ± 0.50 mmol/gprot) and triglyceride (0.88 ± 0.24 mmol/gprot) (p < 0.05). Moreover, L. pentosus CQZC01 elevated the level of interleukin-10 (IL-10; 807 ± 44 pg/mL) but significantly decreased the levels of IL-1β (29.75 ± 5.27pg/mL), IL-6 (58 ± 8 pg/mL), and tumor necrosis factor-α (TNF-α, 564 ± 13 pg/mL). Liver malondialdehyde was also significantly decreased by treatment with L. pentosus CQZC01 from 3.61 ± 0.14 to 2.03 ± 0.49 nmol/mgprot. The relative expression of C-Jun N-terminal kinase, extracellular regulated protein kinases, and cyclooxygenase-1 was downregulated, and the SOD1, SOD2, peroxisome proliferator-activated receptor-α, glutathione peroxidase, catalase, nuclear factor erythroid-2-related factor 2, heme oxygenase-1 and nicotinamide adenine dinucleotide phosphate were upregulated by L. pentosus CQZC01. The overall protective effect of L. pentosus CQZC01 was comparable to commercial Lactobacillus delbrueckii subsp. Bulgaricus. Lactobacillus pentosus CQZC01 might be a suitable hepatoprotective measure for people who frequently ingest alcoholic drinks. PRACTICAL APPLICATION: L. pentosus CQZC01 can alleviate subacute alcoholic liver injury by raising the antioxidant status and upregulating the antioxidant-related genes.
为了降低酒精性肝病的发病率，本研究考察了新分离的戊糖片球菌 CQZC01 对亚急性酒精性肝损伤的保护作用。戊糖片球菌 CQZC01（1×109 CFU/kgbw）经口给药可使小鼠体重保持在 30.54±1.15g；减轻肝形态学损伤；降低透明质酸酶（147±19U/L）、III 型前胶原（4.82±0.54ng/mL）、丙氨酸转氨酶（10.66±2.32U/L）和天冬氨酸转氨酶（15.18±1.98U/L）的活性；增强酒精脱氢酶（65.15±3.2U/mgprot）、醛脱氢酶（16.50±0.96U/mgprot）、超氧化物歧化酶（623±39U/mgprot）和谷胱甘肽（19.54±2.46µmol/gprot）的活性；降低肝总胆固醇（3.59±0.50mmol/gprot）和甘油三酯（0.88±0.24mmol/gprot）（p<0.05）。此外，戊糖片球菌 CQZC01 提高了白细胞介素-10（IL-10；807±44pg/mL）的水平，但显著降低了白细胞介素-1β（29.75±5.27pg/mL）、白细胞介素-6（58±8pg/mL）和肿瘤坏死因子-α（TNF-α，564±13pg/mL）的水平。丙二醛的水平也显著降低，戊糖片球菌 CQZC01 处理后从 3.61±0.14 降低至 2.03±0.49nmol/mgprot。C-Jun N 末端激酶、细胞外调节蛋白激酶和环氧化酶-1 的相对表达下调，而 SOD1、SOD2、过氧化物酶体增殖物激活受体-α、谷胱甘肽过氧化物酶、过氧化氢酶、核因子红细胞相关因子 2、血红素加氧酶-1 和烟酰胺腺嘌呤二核苷酸磷酸的表达上调。戊糖片球菌 CQZC01 的整体保护作用与商业保加利亚乳杆菌相当。戊糖片球菌 CQZC01 可能是一种适合经常饮酒人群的保肝措施。实际应用：戊糖片球菌 CQZC01 通过提高抗氧化状态和上调抗氧化相关基因来缓解亚急性酒精性肝损伤。
