Han Ming-Yang, Lou Wen-Yong, Li Meng-Fan
Food Science School, Guangdong Pharmaceutical University, Zhongshan 528458, China.
Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Foods. 2025 Jul 30;14(15):2690. doi: 10.3390/foods14152690.
Probiotics have been widely explored for their potential in managing hyperuricemia. However, their isolation and identification are fundamental prerequisites for practical application. In this study, 254 lactic acid bacteria (LAB) strains were isolated from Chinese sauerkraut and screened for probiotic potential based on genomic and phenotypic characteristics, as well as nucleoside-degrading activity relevant to decrease serum urate. Among them, () F42 exhibited the highest bile salt tolerance (survivor rate: 19.46 ± 4.33%), strong adhesion to Caco-2 cells (1.89 ± 0.12%), effective nucleoside degradation (inosine: 5.46 ± 0.67 mg∙L∙min; guanosine: 3.84 ± 0.11 mg∙L∙min), and notable anti-listeria activity (inhibition zone: 6.9 ± 0.3 mm). Based on its functional profile, F42 was selected as a promising probiotic candidate for further investigation of its urate-lowering effects. This work provides a new insight into anti-hyperuricemia probiotic selection based on nucleoside-degrading activity.
益生菌在管理高尿酸血症方面的潜力已得到广泛探索。然而，它们的分离和鉴定是实际应用的基本前提。在本研究中，从中国酸菜中分离出254株乳酸菌（LAB）菌株，并根据基因组和表型特征以及与降低血清尿酸相关的核苷降解活性筛选其益生菌潜力。其中，（）F42表现出最高的胆盐耐受性（存活率：19.46±4.33%），对Caco-2细胞的强粘附性（1.89±0.12%），有效的核苷降解（肌苷：5.46±0.67mg∙L∙min；鸟苷：3.84±0.11mg∙L∙min），以及显著的抗李斯特菌活性（抑菌圈：6.9±0.3mm）。基于其功能特性，F42被选为有前景的益生菌候选菌株，以进一步研究其降尿酸作用。这项工作为基于核苷降解活性的抗高尿酸血症益生菌选择提供了新的见解。
