College of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an, 237012, China.
Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, 230012, China.
Curr Pharm Biotechnol. 2023;24(15):1964-1971. doi: 10.2174/1389201024666230418163100.
Sodium new houttuyfonate (SNH) is an adduct of houttuyfonate, which is the main component of the common Chinese medicinal plant . SNH has been widely used in antibacterial and anti-inflammatory treatments in clinics. However, the exact antimicrobial mechanism of SNH is still unclear, despite its mild direct antimicrobial activity Objectives: The aim of this study is to investigate the effect and possible mechanism of SNH on macrophages against bacteria .
In this study, we assessed the antibacterial and anti-inflammatory effects of SNH on the RAW264.7 macrophage cell line against , a major opportunistic pathogen.
Firstly, we found that SNH showed minimal toxicity on RAW264.7 macrophages. Secondly, our results indicated that SNH effectively inhibited the inflammatory reaction of macrophages stimulated by We also found that SNH improved the phagocytosis and killing effect of RAW264.7 macrophages against P. aeruginosa . Furthermore, our results revealed that SNH effectively inhibited the expression of the TLR4/NF-кB pathway in macrophage RAW264.7 co-incubated with .
Based on our findings, SNH can significantly improve the phagocytosis of macrophages and inhibit the excessive release of inflammatory factors by repressing the TLR4/NF-кB pathway.
新鱼腥草素钠（SNH）是鱼腥草素的加合物，鱼腥草素是一种常见中草药的主要成分。SNH 已广泛用于临床抗菌和抗炎治疗。然而，尽管 SNH 具有温和的直接抗菌活性，但它的确切抗菌机制仍不清楚。
本研究旨在探讨 SNH 对巨噬细胞抗细菌的作用及其可能的机制。
在这项研究中，我们评估了 SNH 对 RAW264.7 巨噬细胞系对抗 ，一种主要的机会性病原体的抗菌和抗炎作用。
首先，我们发现 SNH 对 RAW264.7 巨噬细胞的毒性最小。其次，我们的结果表明，SNH 能有效抑制 刺激的巨噬细胞炎症反应。我们还发现 SNH 改善了 RAW264.7 巨噬细胞对铜绿假单胞菌的吞噬和杀伤作用。此外，我们的结果表明，SNH 能有效抑制 TLR4/NF-кB 通路在与 共孵育的巨噬细胞 RAW264.7 中的表达。
根据我们的研究结果，SNH 可通过抑制 TLR4/NF-кB 通路显著改善巨噬细胞的吞噬作用并抑制炎症因子的过度释放。
