Department of Plastic & Reconstructive Surgery, Medical Center Boulevard, Wake Forest University School of Medicine, Winston-Salem, NC, 27157, USA.
Virginia Tech - Wake Forest University School of Biomedical Engineering and Sciences, Winston-Salem, NC, USA.
Ann Biomed Eng. 2024 Jul;52(7):1873-1882. doi: 10.1007/s10439-023-03199-w. Epub 2023 Apr 18.
Blood-biomaterial compatibility is essential for tissue repair especially for endovascular biomaterials where small-diameter vessel patency and endothelium formation is crucial. To address this issue, a composite biomaterial termed PFC fabricated from poly (glycerol sebacate), silk fibroin, and collagen was used to determine if functionalization with syndecan-4 (SYN4) would reduce thrombogenesis through the action of heparan sulfate. The material termed, PFC_SYN4, has structure and composition similar to native arterial tissue and has been reported to facilitate the binding and differentiation of endothelial colony-forming cells (ECFCs). In this study, the hemocompatibility of PFC_SYN4 was evaluated and compared with non-functionalized PFC, electrospun collagen, ePTFE, and bovine pericardial patch (BPV). Ultrastructurally, platelets were less activated when cultured on PFC and PFC_SYN4 compared to collagen where extensive platelet degranulation was observed. Quantitatively, 31% and 44% fewer platelets adhered to PFC_SYN4 compared to non-functionalized PFC and collagen, respectively. Functionalization of PFC resulted in reduced levels of complement activation compared to PFC, collagen, and BPV. Whole blood clotting times indicated that PFC_SYN4 was less thrombogenic compared with PFC, collagen, and BPV. These results suggest that syndecan-4 functionalization of blood-contacting biomaterials provides a novel solution for generating a reduced thrombogenic surface.
血液-生物材料相容性对于组织修复至关重要，特别是对于血管内生物材料，因为小直径血管通畅性和内皮形成至关重要。为了解决这个问题，使用了一种称为 PFC 的复合生物材料，由聚（癸二酸甘油酯）、丝素蛋白和胶原蛋白制成，以确定通过硫酸乙酰肝素的作用对其进行 syndecan-4（SYN4）功能化是否会减少血栓形成。这种称为 PFC_SYN4 的材料具有与天然动脉组织相似的结构和组成，并已被报道可促进内皮祖细胞（ECFC）的结合和分化。在这项研究中，评估了 PFC_SYN4 的血液相容性，并将其与非功能化的 PFC、静电纺丝胶原蛋白、ePTFE 和牛心包补片（BPV）进行了比较。超微结构上，与胶原蛋白相比，血小板在 PFC 和 PFC_SYN4 上的激活程度较低，在胶原蛋白上观察到广泛的血小板脱颗粒。定量分析表明，与非功能化的 PFC 和胶原蛋白相比，PFC_SYN4 上黏附的血小板分别减少了 31%和 44%。与 PFC、胶原蛋白和 BPV 相比，PFC 的补体激活水平降低。全血凝血时间表明，与 PFC、胶原蛋白和 BPV 相比，PFC_SYN4 的血栓形成性较低。这些结果表明，血液接触生物材料的 syndecan-4 功能化提供了一种减少血栓形成表面的新方法。
