Motlagh Delara, Yang Jian, Lui Karen Y, Webb Antonio R, Ameer Guillermo A
Biomedical Engineering Department, Northwestern University, 2145 Sheridan Road, Room E310, Evanston, IL 60208, USA.
Biomaterials. 2006 Aug;27(24):4315-24. doi: 10.1016/j.biomaterials.2006.04.010. Epub 2006 May 3.
Poly(glycerol-sebacate) (PGS) is an elastomeric biodegradable polyester that could potentially be used to engineer blood vessels in vivo. However, its blood-material interactions are unknown. The objectives of this study were to: (a) fabricate PGS-based biphasic tubular scaffolds and (b) assess the blood compatibility of PGS in vitro in order to get some insight into its potential use in vivo. PGS was incorporated into biphasic scaffolds by dip-coating glass rods with PGS pre-polymer. The thrombogenicity (platelet adhesion and aggregation) and inflammatory potential (IL-1beta and TNFalpha expression) of PGS were evaluated using fresh human blood and a human monocyte cell line (THP-1). The activation of the clotting system was assessed via measurement of tissue factor expression on THP-1 cells, plasma recalcification times, and whole blood clotting times. Glass, tissue culture plastic (TCP), poly(l-lactide-co-glycolide) (PLGA), and expanded polytetrafluorethylene (ePTFE) were used as reference materials. Biphasic scaffolds with PGS as the blood-contacting surface were successfully fabricated. Relative to glass (100%), platelet attachment on ePTFE, PLGA and PGS was 61%, 100%, and 28%, respectively. PGS elicited a significantly lower release of IL-1beta and TNFalpha from THP-1 cells than ePTFE and PLGA. Similarly, relative to all reference materials, tissue factor expression by THP-1 cells was decreased when exposed to PGS. Plasma recalcification and whole blood clotting profiles of PGS were comparable to or better than those of the reference polymers tested.
聚（甘油-癸二酸酯）（PGS）是一种可生物降解的弹性体聚酯，有可能用于体内血管工程。然而，其血液与材料的相互作用尚不清楚。本研究的目的是：（a）制备基于PGS的双相管状支架，以及（b）在体外评估PGS的血液相容性，以便深入了解其在体内的潜在用途。通过用PGS预聚物浸涂玻璃棒将PGS掺入双相支架中。使用新鲜人血和人单核细胞系（THP-1）评估PGS的血栓形成性（血小板粘附和聚集）和炎症潜能（IL-1β和TNFα表达）。通过测量THP-1细胞上组织因子的表达、血浆复钙时间和全血凝血时间来评估凝血系统的激活情况。玻璃、组织培养塑料（TCP）、聚（L-丙交酯-共-乙交酯）（PLGA）和膨体聚四氟乙烯（ePTFE）用作参考材料。成功制备了以PGS为血液接触表面的双相支架。相对于玻璃（100%），ePTFE、PLGA和PGS上的血小板附着率分别为61%、100%和28%。与ePTFE和PLGA相比，PGS从THP-1细胞中引发的IL-1β和TNFα释放显著降低。同样，相对于所有参考材料，当THP-1细胞暴露于PGS时，其组织因子表达降低。PGS的血浆复钙和全血凝血情况与所测试的参考聚合物相当或更好。
