Key Laboratory of Molecular Medicine and Biotherapy in the Ministry of Industry and Information Technology, School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
China Meat Research Center, Beijing 100068, PR China.
ACS Appl Mater Interfaces. 2023 May 3;15(17):20788-20799. doi: 10.1021/acsami.3c02420. Epub 2023 Apr 18.
Au nanozymes are extensively researched for their photothermal effect and catalytic performance, but overcoming the inherent defects of poor dispersibility and thermal stability through complementary materials will expand their prospects for biological applications. Herein, several novel CAu nanozymes were fabricated by in situ reduction of chloroauric acid on hollow carbon nanospheres (HCNs). Through regulating the number of reductions, sesame ball-shaped CAu (sCAu) with highly dispersed Au nanoparticles and diversity-shaped CAu (dCAu) were obtained. The number and morphology of loaded Au nanoparticles, absorption spectra, and hydrophilicity of CAu nanozymes were systematically characterized to demonstrate the flexibility of this novel method. The high-efficiency peroxidase-like sCAu0.3 nanozyme with hyperthermia-activated property was then screened for later bio-application. It is worth mentioning that its photothermal-promoted peroxidase-like activity could be achieved under near-infrared laser irradiation. Moreover, sCAu0.3 could specifically achieve glutathione detection in human blood samples. This method will provide a protocol for the regulation of CAu nanozymes to adapt to bio-detection applications.
金纳米酶因其光热效应和催化性能而被广泛研究，但通过互补材料克服其固有缺陷，如分散性差和热稳定性差，将扩大其在生物应用中的前景。本文通过在中空碳纳米球（HCNs）上原位还原氯金酸制备了几种新型 CAu 纳米酶。通过调节还原次数，得到了高度分散的金纳米粒子的芝麻球状 CAu（sCAu）和多样性形状的 CAu（dCAu）。系统地表征了 CAu 纳米酶的载金纳米粒子数量和形态、吸收光谱和亲水性，以证明这种新方法的灵活性。然后筛选出具有高热激活特性的高效过氧化物酶样 sCAu0.3 纳米酶用于后续的生物应用。值得一提的是，它的光热促进过氧化物酶样活性可以在近红外激光照射下实现。此外，sCAu0.3 可以特异性地检测人血样中的谷胱甘肽。该方法将为调节 CAu 纳米酶以适应生物检测应用提供一种方案。
