Reidy M A, Yoshida K, Harker L A, Schwartz S M
Arteriosclerosis. 1986 May-Jun;6(3):305-11. doi: 10.1161/01.atv.6.3.305.
Rat platelets were labeled with indium-111 (111In) and injected into rats whose aortas were subjected to endothelial denudation. A plot of 111In activity vs the number of missing endothelial cells showed a direct correlation between these parameters and it was possible to detect endothelial denudation when approximately 600 cells were removed. These studies also revealed that intact adjacent endothelium from aortas subjected to injury had detectably increased 111In activity compared with endothelium of control, uninjured rats. This activity was not found if 111In-platelets were preincubated with acetylsalicylic acid (1.3 mM) prior to injection and subsequent denudation procedure. To test if 111In platelet activity could be transported to sites distant from the site of injury, a small catheter (PE 50) was passed down the carotid artery so that it reached the aortic arch or inserted via a femoral artery to the iliac-aortic bifurcation. Significant increases in 111In activity were detected in the lower thoracic aorta when the catheter was introduced into the aortic arch but not when the catheter was introduced via the femoral artery. Morphologic evaluation of these sites showed no endothelial cell loss or adhering platelets. These studies suggest that detectable uptake of released platelet products may occur at distal sites of intact endothelium.
将铟 - 111（¹¹¹In）标记的大鼠血小板注入主动脉内皮剥脱的大鼠体内。¹¹¹In活性与缺失内皮细胞数量的关系图显示，这些参数之间存在直接相关性，当去除约600个细胞时能够检测到内皮剥脱。这些研究还表明，与未受伤的对照大鼠的内皮相比，遭受损伤的主动脉完整相邻内皮的¹¹¹In活性有可检测到的增加。如果¹¹¹In标记的血小板在注射及后续剥脱操作前与乙酰水杨酸（1.3 mM）预孵育，则不会出现这种活性。为了测试¹¹¹In血小板活性是否能转运到远离损伤部位的位点，将一根小导管（PE 50）沿颈动脉插入，使其到达主动脉弓，或经股动脉插入至髂 - 主动脉分叉处。当导管插入主动脉弓时，在胸主动脉下段检测到¹¹¹In活性显著增加，但经股动脉插入导管时则未检测到。对这些位点的形态学评估显示没有内皮细胞丢失或血小板黏附。这些研究表明，在完整内皮的远端位点可能会出现可检测到的释放血小板产物摄取。
