Bronnec Pauline, Sousa Jeremy, Henry Thomas
CIRI, Centre International de Recherche en Infectiologie, Inserm U1111, Univ Lyon, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, Lyon, France.
Methods Mol Biol. 2023;2641:37-47. doi: 10.1007/978-1-0716-3040-2_4.
The pyrin inflammasome detects bacterial toxins and effectors that inhibit RhoA GTPases and triggers inflammatory cytokine release and a fast cell death termed pyroptosis. In addition, various endogenous molecules, drugs, synthetic molecules, or mutations can trigger pyrin inflammasome activation. The pyrin protein differs between humans and mice, and the repertoire of pyrin activators is also species-specific. Here, we present the various pyrin inflammasome activators, inhibitors, the kinetics of pyrin activation in response to the various activators, and their species specificity. In addition, we present different methods to monitor pyrin-mediated pyroptosis.
吡啉炎性小体可检测抑制RhoA GTP酶的细菌毒素和效应蛋白，并触发炎性细胞因子释放以及一种称为细胞焦亡的快速细胞死亡。此外，各种内源性分子、药物、合成分子或突变均可触发吡啉炎性小体激活。吡啉蛋白在人和小鼠之间存在差异，吡啉激活剂的种类也具有物种特异性。在此，我们介绍了各种吡啉炎性小体激活剂、抑制剂、响应各种激活剂时吡啉激活的动力学及其物种特异性。此外，我们还介绍了监测吡啉介导的细胞焦亡的不同方法。
