Heller Allie, Du Fang, Liu Yongqiang, Yang Yijun, Yang Zeng-Jie
Nuclear Dynamics and Cancer Program, Fox Chase Cancer Center, Temple University Health System, Philadelphia, PA 19111, USA.
Nuclear Dynamics and Cancer Program, Fox Chase Cancer Center, Temple University Health System, Philadelphia, PA 19111, USA.
STAR Protoc. 2023 Apr 18;4(2):102234. doi: 10.1016/j.xpro.2023.102234.
Tumor relapse is the leading adverse prognostic factor in medulloblastoma (MB). However, there is still no established mouse model for MB relapse, impeding our efforts to develop strategies to treat relapsed MB. We present a protocol for generating a mouse model for relapsed MB using irradiation by optimizing mouse breeding and age, as well as irradiation dosage and timing. We then detail procedures for determining tumor relapse based on tumor cell trans-differentiation in MB tissue, immunohistochemistry, and tumor cell isolation. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021)..
肿瘤复发是髓母细胞瘤（MB）的主要不良预后因素。然而，目前仍没有已建立的MB复发小鼠模型，这阻碍了我们开发治疗复发性MB策略的努力。我们提出了一种通过优化小鼠繁殖、年龄以及辐射剂量和时间，利用辐射生成复发性MB小鼠模型的方案。然后，我们详细介绍了基于MB组织中的肿瘤细胞转分化、免疫组织化学和肿瘤细胞分离来确定肿瘤复发的程序。有关本方案使用和执行的完整详细信息，请参考郭等人（2021年）的研究。
