Cornish-Bowden A, Endrenyi L
Biochem J. 1986 Feb 15;234(1):21-9. doi: 10.1042/bj2340021.
A method described previously [Cornish-Bowden & Endrenyi (1981) Biochem. J. 193, 1005-1008] for fitting theoretical equations to enzyme kinetic data without prior knowledge of weights or error distribution has been tested by computer simulation. With the equations for various kinds of linear inhibition as an example, the method performed well under all of the conditions examined, giving results that were often much better than those given by widely used least-squares alternatives, and were never appreciably worse. Although equations for two-substrate kinetics were not explicitly tested, the results for inhibition equations can be generalized to include two-substrate equations because the two are formally equivalent for simulation purposes. As a check on the results with inhibition equations the method was also tested for fitting bell-shaped pH-activity profiles and gave correspondingly good results.
之前描述的一种方法[康沃尔-鲍登和恩德里尼（1981年），《生物化学杂志》193卷，1005 - 1008页]，用于在无需事先了解权重或误差分布的情况下将理论方程拟合到酶动力学数据，已通过计算机模拟进行了测试。以各种线性抑制的方程为例，该方法在所研究的所有条件下都表现良好，得出的结果通常比广泛使用的最小二乘法替代方法给出的结果要好得多，而且从未明显更差。虽然未明确测试双底物动力学方程，但抑制方程的结果可以推广到包括双底物方程，因为出于模拟目的，两者在形式上是等效的。作为对抑制方程结果的检验，该方法还针对钟形pH - 活性曲线的拟合进行了测试，并得出了相应的良好结果。
