Cornish-Bowden A, Eisenthal R
Biochim Biophys Acta. 1978 Mar 14;523(1):268-72. doi: 10.1016/0005-2744(78)90030-x.
When estimates of Michaelis-Menten parameters are obtained from kinetic observations taken in pairs, as in the direct linear plot, bias can arise in the final estimates if any pairs lead to negative values of the maximum velocity V. This bias can be removed by treating such negative values as if they were large and positive, and by treating the corresponding values of Km in the same way.
当像在直接线性图中那样从成对进行的动力学观测中获得米氏参数的估计值时，如果任何一对观测导致最大速度V出现负值，那么最终估计值可能会产生偏差。通过将这些负值当作大的正值来处理，并对Km的相应值也进行同样的处理，可以消除这种偏差。
