Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Ankerui (Shanxi) Biological Cell Co., Ltd. , Shanxi, 030000, China.
Adv Healthc Mater. 2023 Jul;12(19):e2202638. doi: 10.1002/adhm.202202638. Epub 2023 May 1.
Ischemic stroke is a primary vascular disease of the central nervous system characterized by high morbidity, mortality, and healthcare costs. As conventional ischemic stroke models fail to predict therapeutic efficacy, in vitro neurovascular unit (NVU)/blood-brain barrier (BBB) models are utilized to model ischemic stroke through replicating the cell-cell interactions and mimicking the blood flow and anatomical features of the brain. Here, an overview of transwell, microfluidic, and hydrogel-based NVU/BBB models is provided, including cell types, engineering approaches, and the simulation of physiological and pathological features of NVU/BBB after ischemic stroke. Collectively, recent advances in 3D-printed NVU models are emphasized, which are anticipated to be a promising system for more reliable mechanistic studies and preclinical drug screenings that can eventually accelerate the drug development process for the ischemic stroke therapy.
缺血性脑卒中是一种中枢神经系统的主要血管疾病，其特点是发病率、死亡率和医疗保健费用高。由于传统的缺血性脑卒中模型无法预测治疗效果，因此利用体外血脑屏障（BBB）/神经血管单元（NVU）模型通过复制细胞间相互作用并模拟大脑的血流和解剖特征来模拟缺血性脑卒中。本文概述了基于 Transwell、微流控和水凝胶的 NVU/BBB 模型，包括细胞类型、工程方法以及缺血性脑卒中后 NVU/BBB 的生理和病理特征的模拟。总之，强调了 3D 打印 NVU 模型的最新进展，预计该模型将成为更可靠的机制研究和临床前药物筛选的有前途的系统，最终加速缺血性脑卒中治疗的药物开发过程。
