Löber G, Kittler L, Beensen V, Schaarschmidt H, Knopf B
Biomed Biochim Acta. 1986;45(3):343-51.
The effect of 8-methoxypsoralen (8-MOP, 5 X 10(-5) M), near UV-light of 365 wavelength (UVA, 1.5 J/cm2) and the combination of both (PUVA treatment) were studied on lymphocytes in vitro taken from healthy persons and patients with psoriasis vulgaris and psoriasis arthritis (psoriasis arthropathica). Chromosomes isolated from cell nuclei were visualized by means of Giemsa staining technique and analyzed for induction of chromosomal defects, i.e. premature centromere division (PCD), major coiling (MC), and formation of gaps and fragile sites. Exposure of nonpsoriatic lymphocytes to 8-MOP, UVA or PUVA increased the rate of PCD or MC generation. In experiments with psoriatic lymphocytes a much weaker effect was found, with a moderate increase of PCD and MC after UVA or PUVA treatment in the case of psoriasis vulgaris, and of MC after UVA treatment of psoriasis arthritis. On the average the number of chromosomes per metaphase plate displaying PCD did not exceed 10. No indication was obtained for the preference of certain chromosome groups or the appearance of "fragile sites". Under all experimental conditions the number of chromosome gaps ranged in the order of their spontaneous induction. Our findings suggest PCD and MC investigations as possible sensitive tools for diagnosing latent psoriasis and for refined analysis of psoriatic cells or chromosomes. However, more experiments along this line are needed.
研究了8-甲氧基补骨脂素（8-MOP，5×10⁻⁵ M）、365波长的近紫外光（UVA，1.5 J/cm²）以及两者联合使用（PUVA疗法）对从健康人、寻常型银屑病患者和银屑病关节炎（关节病型银屑病）患者体内获取的淋巴细胞的体外作用。通过吉姆萨染色技术对从细胞核中分离出的染色体进行可视化处理，并分析染色体缺陷的诱导情况，即着丝粒过早分裂（PCD）、大螺旋化（MC）以及间隙和脆性位点的形成。非银屑病淋巴细胞暴露于8-MOP、UVA或PUVA会增加PCD或MC的产生率。在银屑病淋巴细胞的实验中，发现作用要弱得多，寻常型银屑病患者经UVA或PUVA处理后PCD和MC有适度增加，关节病型银屑病患者经UVA处理后MC增加。平均而言，每个中期板显示PCD的染色体数量不超过10条。未发现某些染色体组有偏好或出现“脆性位点”的迹象。在所有实验条件下，染色体间隙的数量按其自发诱导的顺序排列。我们的研究结果表明，PCD和MC研究可能是诊断潜伏性银屑病以及对银屑病细胞或染色体进行精细分析的敏感工具。然而，还需要沿着这条线进行更多实验。
