Department of Biotechnology, College of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, Chennai - 603203, Tamil Nadu, India.
Department of Biochemistry, College of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, Chennai - 603203, Tamil Nadu, India.
Tissue Cell. 2023 Jun;82:102092. doi: 10.1016/j.tice.2023.102092. Epub 2023 Apr 13.
Mesenchymal stem cells (MSCs) are being used in clinical trials given their proliferative potential, immunomodulatory effects, and their pro-angiogenic, anti-apoptotic, and anti-fibrotic properties. Umbilical cord tissue is an excellent source of MSCs. Iron-fortified calf serum is being used to culture MSCs as an alternative to fetal bovine serum since it is relatively inexpensive. Fetal calf serum is fortified with iron since calves are often fed a low-iron diet. However, the use of iron-fortified calf serum is still problematic since it is xenogeneic. Lately, human platelet lysate is being used for the culture of human cells. To increase its shelf life, human platelet lysate was lyophilized and used for culturing human umbilical cord tissue mesenchymal stem cells (hUCT-MSCs). This study compares the culture of hUCT-MSCs with either iron-fortified calf serum or lyophilized human platelet lysate (LHPL). Trilineage differentiation potential (for chondrogenesis, adipogenesis, or osteogenesis) was assessed and immunomodulatory properties of hUCT-MSC were studied using the Mixed Lymphocyte Reaction (MLR) to detect inhibition of the proliferation of lymphocytes. This study concludes the potency of LHPL as the best alternative to Iron-Fortified Calf Serum (IFCS) for culture expansion of hUCT-MSC. hUCT-MSC cultured with LHPL display characteristic surface markers and have the capacity for trilineage differentiation.
间充质干细胞（MSCs）因其增殖潜能、免疫调节作用以及促血管生成、抗细胞凋亡和抗纤维化特性而被用于临床试验。脐带组织是 MSCs 的极佳来源。由于相对便宜，铁强化小牛血清被用于培养 MSCs，以替代胎牛血清。小牛通常喂食低铁饮食，因此胎牛血清中添加了铁。然而，由于铁强化小牛血清是异种来源的，因此其使用仍然存在问题。最近，人血小板裂解液被用于培养人细胞。为了延长其保质期，将人血小板裂解液冻干并用于培养人脐带组织间充质干细胞（hUCT-MSCs）。本研究比较了铁强化小牛血清或冻干人血小板裂解液（LHPL）培养 hUCT-MSCs 的效果。通过三系分化潜能（用于软骨形成、脂肪形成或成骨）评估和混合淋巴细胞反应（MLR）研究 hUCT-MSC 的免疫调节特性，以检测淋巴细胞增殖的抑制作用。本研究得出结论，LHPL 是替代铁强化小牛血清（IFCS）培养 hUCT-MSC 的最佳选择。用 LHPL 培养的 hUCT-MSC 显示出特征性的表面标志物，并具有三系分化的能力。
