College of Life Science and Bioengineering, Beijing University of Technology, Beijing, 100124, China.
In Vitro Cell Dev Biol Anim. 2013 Dec;49(10):771-7. doi: 10.1007/s11626-013-9663-8. Epub 2013 Sep 17.
Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) hold great potential for their therapeutic use in various clinical diseases. Many publications have reported on human blood-derived alternatives to animal serum for culturing mesenchymal stem cells, such as human serum, allogenic umbilical cord blood serum, and human platelet derivatives. However, it is not clear whether human umbilical cord blood plasma (UCBP), as the surplusage of umbilical cord blood mesenchymal stem cell extraction, could be used. In this study, in order to make the best of umbilical cord blood, the human UCBP was dialyzed to replace fetal bovine serum (FBS) in the culture medium. hUC-MSCs were cultured in the new medium. Cell growth rate, specific biomarkers, and differentiation properties were detected to characterize the cell proliferation and MSC-specific properties. The hUC-MSCs cultured in such derived medium were verified with proliferation rate, cluster differentiation markers, cell cycle, as well as differentiation capabilities. Such dialyzed human UCBP is fully comparable with, if not superior to, FBS in deriving and culturing hUC-MSCs.
人脐带间充质干细胞（hUC-MSCs）在治疗各种临床疾病方面具有巨大的应用潜力。许多出版物都报道了用人血替代动物血清来培养间充质干细胞，如人血清、异体脐带血血清和人血小板衍生物。然而，尚不清楚作为脐带间充质干细胞提取的剩余物的人脐带血血浆（UCBP）是否可以使用。在这项研究中，为了充分利用脐带血，用人 UCBP 透析液替代培养基中的胎牛血清（FBS）。在新的培养基中培养 hUC-MSCs。检测细胞生长速度、特异性生物标志物和分化特性，以表征细胞增殖和 MSC 特异性。通过增殖率、簇分化标志物、细胞周期以及分化能力对在这种衍生培养基中培养的 hUC-MSCs 进行了验证。这种透析后的人 UCBP 在诱导和培养 hUC-MSCs 方面与 FBS 完全可比，如果不是优于 FBS 的话。
