Sandberg L B, Wolt T B, Leslie J G
Biochem Biophys Res Commun. 1986 Apr 29;136(2):672-8. doi: 10.1016/0006-291x(86)90493-6.
Digestion of insoluble porcine elastin with thermolysin produces a number of discrete small peptides. That present in highest concentration is the pentapeptide valyl-glycyl-valyl-prolyl-glycine (VGVPG) derived from the portion of the polymer containing extensive repeats of this sequence. Among eukaryotes, this sequence appears to be found only in elastin and its precursor tropoelastin. In the pig this is represented by peptide W4 of a tropoelastin tryptic digest (Sandberg, L.B., et al. Path. Biol. 33, 266-274, 1985). Quantitation of this peptide by HPLC separation, monitoring its absorption at 212 nm, offers a simple reliable means of measuring purified insoluble elastin as well as non-purified elastin in fat-free tissue samples. Digestion times and incubation temperatures are discussed. The method is sensitive enough to accurately quantitate elastin at the 2 to 3 microgram level.
用嗜热菌蛋白酶消化不溶性猪弹性蛋白可产生许多离散的小肽。浓度最高的是五肽缬氨酰-甘氨酰-缬氨酰-脯氨酰-甘氨酸（VGVPG），它来源于聚合物中含有该序列大量重复的部分。在真核生物中，该序列似乎仅存在于弹性蛋白及其前体原弹性蛋白中。在猪中，这由原弹性蛋白胰蛋白酶消化产物的肽W4代表（桑德伯格，L.B.等人，《病理生物学》33，266 - 274，1985）。通过高效液相色谱分离对该肽进行定量，监测其在212nm处的吸光度，为测量无脂肪组织样品中的纯化不溶性弹性蛋白以及未纯化的弹性蛋白提供了一种简单可靠的方法。文中讨论了消化时间和孵育温度。该方法灵敏度足够高，能够准确地对2至3微克水平的弹性蛋白进行定量。
