Price L S, Roos P J, Shively V P, Sandberg L B
Department of Biochemistry, Loma Linda University CA 92357.
Matrix. 1993 Jul;13(4):307-11. doi: 10.1016/s0934-8832(11)80026-0.
Thermolysin digests of human elastins were examined for reliable elastin peptide markers as determined by HPLC followed by amino acid sequencing of promising peaks. The tetrapeptide VAPG was found to occur in the early portion of the chromatogram in a highly reliable fashion. The peptide appears to be significantly amplified, when compared with the other peptides, in that it is derived from the hexapeptide repeat in elastin, VGVAPG, which repeats itself in two three-piece segments in the c-terminal portion of the tropoelastin molecule. VAPG serves as a highly reliable quantitative measure for human elastins, allowing sensitivities to less than a microgram. Thus, it is a significantly more accurate measure than other existing methods. Precision also appears to be enhanced because of the directness of the measurement. The use of VAPG as a quantitative marker for human elastin has clinical application in the study of elastin-based connective tissue diseases.
对人弹性蛋白的嗜热菌蛋白酶消化产物进行检测，以寻找可靠的弹性蛋白肽标志物，方法是通过高效液相色谱（HPLC），然后对有前景的峰进行氨基酸测序。发现四肽VAPG以高度可靠的方式出现在色谱图的早期部分。与其他肽相比，该肽似乎有显著扩增，因为它源自弹性蛋白中的六肽重复序列VGVAPG，该序列在原弹性蛋白分子的c末端部分以两个三段片段的形式重复出现。VAPG可作为人弹性蛋白高度可靠的定量指标，灵敏度可达微克以下。因此，它是一种比其他现有方法显著更准确的测量方法。由于测量的直接性，精密度似乎也有所提高。将VAPG用作人弹性蛋白的定量标志物在基于弹性蛋白的结缔组织疾病研究中有临床应用。
