Okuyama A, Ohuchi S, Tanaka T, Aoyagi T, Umezawa H
Biochem Int. 1986 Mar;12(3):485-91.
Arphamenine A was synthesized in a cell-free system obtained from the arphamenine-producing strain, Chromobacterium violaceum BMG361-CF4. L-[14C]-phenylalanine was converted to beta-phenylpyruvic acid by phenylalanine amino-transferase obtained from the 10,000 x g supernatant (S10 fraction). [14C]-Benzylmalic acid was synthesized from beta-phenylpyruvic acid with [14C]-acetyl-CoA in the S10 fraction. [14C]-Benzylsuccinic acid was formed from beta-phenylpyruvic acid with [14C]-acetyl-CoA and ATP in this fraction, as was [14C]-arphamenine A from benzylsuccinic acid and L-[14C]-arginine. Thus, the pathway of arphamenine A biosynthesis was confirmed by the cell-free biosynthesis of this antibiotic.
从产阿弗门汀的菌株紫色色杆菌BMG361 - CF4获得的无细胞体系中合成了阿弗门汀A。通过从10,000×g上清液（S10组分）中获得的苯丙氨酸氨基转移酶，L-[14C]-苯丙氨酸被转化为β-苯丙酮酸。在S10组分中，[14C]-苄基苹果酸由β-苯丙酮酸与[14C]-乙酰辅酶A合成。在该组分中，[14C]-苄基琥珀酸由β-苯丙酮酸与[14C]-乙酰辅酶A和ATP形成，[14C]-阿弗门汀A由苄基琥珀酸和L-[14C]-精氨酸形成。因此，通过该抗生素的无细胞生物合成证实了阿弗门汀A的生物合成途径。
