Harman A W, McCamish L E
Biochem Pharmacol. 1986 May 15;35(10):1731-5. doi: 10.1016/0006-2952(86)90331-x.
Hepatocytes from postnatal and adult mice were isolated by perfusion of the liver with a collagenase-containing bicarbonate buffer. These were allowed to attach to collagen-coated tissue culture dishes and were then examined for their susceptibility to paracetamol toxicity. After an 8 hr incubation in either 0.1 or 1.0 mM paracetamol, the extent of lactate dehydrogenase leakage and depletion of glutathione were similar in hepatocytes from young (1-, 2- and 3-week-old) mice when compared to adult mice. The covalent binding of [14C]-paracetamol to protein was greater in the hepatocytes from young mice. The results indicate that while the amount of reactive metabolites free to react with cellular constituents is greater in hepatocytes from young mice, the amount of damage produced was not different than that found in those from adults.
通过用含胶原酶的碳酸氢盐缓冲液灌注肝脏，分离出生后和成年小鼠的肝细胞。将这些细胞接种到包被有胶原蛋白的组织培养皿上，然后检测它们对扑热息痛毒性的易感性。在0.1或1.0 mM扑热息痛中孵育8小时后，与成年小鼠相比，幼年（1、2和3周龄）小鼠肝细胞中乳酸脱氢酶泄漏程度和谷胱甘肽消耗情况相似。[14C] - 扑热息痛与蛋白质的共价结合在幼年小鼠肝细胞中更高。结果表明，虽然幼年小鼠肝细胞中可与细胞成分发生反应的活性代谢物数量更多，但所产生的损伤量与成年小鼠肝细胞中的损伤量并无差异。
