State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Plant Cell Rep. 2023 Jun;42(6):1039-1057. doi: 10.1007/s00299-023-03011-y. Epub 2023 Apr 20.
Common loci and candidate genes for controlling salt-alkali tolerance and yield-related traits were identified in Brassica napus combining QTL mapping with transcriptome under salt and alkaline stresses. The yield of rapeseed (Brassica napus L.) is determined by multiple yield-related traits, which are susceptible to environmental factors. Many yield-related quantitative trait loci (QTLs) have been reported in Brassica napus; however, no studies have been conducted to investigate both salt-alkali tolerance and yield-related traits simultaneously. Here, specific-locus amplified fragment sequencing (SLAF-seq) technologies were utilized to map the QTLs for salt-alkali tolerance and yield-related traits. A total of 65 QTLs were identified, including 30 QTLs for salt-alkali tolerance traits and 35 QTLs for yield-related traits, accounting for 7.61-27.84% of the total phenotypic variations. Among these QTLs, 18 unique QTLs controlling two to four traits were identified by meta-analysis. Six novel and unique QTLs were detected for salt-alkali tolerance traits. By comparing these unique QTLs for salt-alkali tolerance traits with those previously reported QTLs for yield-related traits, seven co-localized chromosomal regions were identified on A09 and A10. Combining QTL mapping with transcriptome of two parents under salt and alkaline stresses, thirteen genes were identified as the candidates controlling both salt-alkali tolerance and yield. These findings provide useful information for future breeding of high-yield cultivars resistant to alkaline and salt stresses.
利用 QTL 作图和盐碱性胁迫下的转录组联合分析鉴定甘蓝型油菜耐盐碱性和产量相关性状的共同位点和候选基因。油菜（甘蓝型油菜）的产量由多个产量相关性状决定，这些性状易受环境因素的影响。已在甘蓝型油菜中报道了许多与产量相关的数量性状位点（QTL），但尚未研究同时耐盐碱性和产量相关性状。在这里，利用特异区域扩增片段测序（SLAF-seq）技术对耐盐碱性和产量相关性状的 QTL 进行了作图。共鉴定到 65 个 QTL，包括 30 个耐盐碱性性状 QTL 和 35 个产量相关性状 QTL，占总表型变异的 7.61-27.84%。在这些 QTL 中，通过荟萃分析鉴定到了 18 个控制两个到四个性状的独特 QTL。检测到 6 个耐盐碱性新的独特 QTL。通过将这些耐盐碱性独特 QTL 与先前报道的产量相关性状的 QTL 进行比较，在 A09 和 A10 上鉴定到了七个共定位的染色体区域。将 QTL 作图与盐碱性胁迫下两个亲本的转录组相结合，鉴定到了 13 个可能同时控制耐盐碱性和产量的候选基因。这些发现为未来培育耐碱性和盐胁迫的高产品种提供了有用的信息。
