Zhou Fei, Liu Yan, Liang Chunbo, Wang Wenjun, Li Cen, Guo Yongli, Ma Jun, Yu Ying, Fan Lijuan, Yao Yubo, Zhao Dongsheng, Liu Xuemei, Huang Xutang
College of Life Science, Northeast Forestry University, Harbin, 150040, China.
Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, China.
Breed Sci. 2018 Dec;68(5):596-605. doi: 10.1270/jsbbs.18051. Epub 2018 Nov 21.
High-density genetic linkage maps are particularly important for quantitative trait loci (QTL) mapping, genome assembly, and marker-assisted selection (MAS) in plants. In this study, a high-density genetic linkage map of sunflower ( L.) was constructed using an F population generated from a cross between L. '86-1' and 'L-1-OL-1' via specific-locus amplified fragment sequencing (SLAF-seq). After sequence preprocessing, 530.50 M reads (105.60 Gb) were obtained that contained a total of 343,197 SLAFs, of which 39,589 were polymorphic. Of the polymorphic SLAFs, 6,136 were organized into a linkage map consisting of 17 linkage groups (LGs) spanning 2,221.86 cM, with an average genetic distance of 0.36 cM between SLAFs. Based on this high-density genetic map, QTL analysis was performed that focused on four sunflower phenotypic traits: oleic acid content (OAC), plant height (PH), head diameter (HD), and stem diameter (SD). Subsequently, for these four traits eight QTLs were detected that will likely be useful for increasing our understanding of genetic factors underlying these traits and for use in marker-assisted selection (MAS) for future sunflower breeding.
高密度遗传连锁图谱对于植物的数量性状基因座（QTL）定位、基因组组装和标记辅助选择（MAS）尤为重要。在本研究中，通过特异位点扩增片段测序（SLAF-seq），利用由向日葵‘86 - 1’和‘L - 1 - OL - 1’杂交产生的F群体构建了向日葵高密度遗传连锁图谱。经过序列预处理，获得了530.50 M条 reads（105.60 Gb），共包含343,197个SLAF，其中39,589个具有多态性。在这些多态性SLAF中，6,136个被构建成一个由17个连锁群（LG）组成的连锁图谱，跨度为2,221.86 cM，SLAF之间的平均遗传距离为0.36 cM。基于此高密度遗传图谱，对四个向日葵表型性状进行了QTL分析：油酸含量（OAC）、株高（PH）、花盘直径（HD）和茎直径（SD）。随后，针对这四个性状检测到8个QTL，这可能有助于增进我们对这些性状潜在遗传因素的理解，并用于未来向日葵育种的标记辅助选择（MAS）。
